Methods of making pancreatic islet cells
First Claim
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1. A method of obtaining pancreatic islet cells, the method comprising:
- providing differentiated pancreatic duct or exocrine cells substantially free of islet cells, allowing said differentiated duct or exocrine cells to proliferate to form a population of dedifferentiated pancreatic cells;
adding a component of extracellular matrix (ECM) to the population of dedifferentiated pancreatic cells; and
growing the cells in the presence of the component of ECM for a time sufficient for the dedifferentiated cells to express insulin, thereby obtaining pancreatic islet cells.
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Abstract
The invention features methods of promoting dedifferentiation of pancreatic cells, methods of obtaining pancreatic islet cells from the dedifferentiated pancreatic cells, and methods of treating a subject having a disorder characterized by insufficient pancreatic islet function by administering pancreatic islet cells obtained by these methods.
104 Citations
36 Claims
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1. A method of obtaining pancreatic islet cells, the method comprising:
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providing differentiated pancreatic duct or exocrine cells substantially free of islet cells, allowing said differentiated duct or exocrine cells to proliferate to form a population of dedifferentiated pancreatic cells;
adding a component of extracellular matrix (ECM) to the population of dedifferentiated pancreatic cells; and
growing the cells in the presence of the component of ECM for a time sufficient for the dedifferentiated cells to express insulin, thereby obtaining pancreatic islet cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 27, 28, 29, 30, 31, 32, 33)
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12. A method of obtaining pancreatic islet cells, the method comprising:
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(a) obtaining a population of adult or differentiated pancreatic cells substantially free of islet cells, (b) allowing the adult or differentiated pancreatic cells to proliferate to form a population of dedifferentiated pancreatic cells;
(c) adding a component of extracellular matrix (ECM) to the population of dedifferentiated pancreatic cells; and
(d) growing the cells in the presence of the component of ECM for a time sufficient for the dedifferentiated cells to express insulin, thereby obtaining pancreatic islet cells. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 34, 35, 36)
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Specification