Methods for manufacturing bioelectronic devices

• US 6,815,218 B1
• Filed: 06/08/2000
• Issued: 11/09/2004
• Est. Priority Date: 06/09/1999
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Ridley, Brent, Jacobson, Joseph M., Manalis, Scott
• Primary Examiner(s): Coleman, W. David
• Application Number:
  US09/590,044
• Time in Patent Office: 1,615 Days
• Field of Search: 438/1, 438/49, 438/800, 438/8, 257/40, 257/252, 257/253, 257/414
• US Class Current: 438/1
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00731 Saccharides
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 40/12 Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: G01N 33/54346 Nanoparticles

Abstract

Bioelectronic components are formed using nanoparticles surrounded by attached shells of at least one biological material. The nanoparticles are deposited (e.g., using a printing process) onto a surface, and by associating the deposited nanoparticles with one or more electrical contacts, electrical measurement across the nanoparticles (and, consequently, across the biological material) may be made. A finished component may include multiple layers formed by nanoparticle deposition.

Claims

1. A method of fabricating a bioelectronic component, the method comprising the steps of:

a. providing a batch of nanoparticles having submicron sizes and an electrical characteristic;

b. attaching at least one biological material to the nanoparticles so as to form shells of the biological material therearound, wherein the biological material is selected from the group consisting of proteins, polypeptides, nucleic acids, polysaccarides, carbohydrates, enzyme substrates, antigens, antibodies, pharmaceuticals, and combinations thereof;

c. depositing onto a surface the nanoparticles coated with shells attached thereto; and

d. causing the deposited nanoparticles to be in electrical communication with at least one electrical contact to facilitate an electrical measurement thereof, the electrical measurement being affected by the biological material.

2. The method of claim 1 in which the nanoparticles are caused to be in electrical communication with said electrical contact by self-assembly.

3. The method of claim 1 in which the nanoparticles are caused to be in electrical communication with said electrical contact by electrostatic assembly.

4. The method of claim 1 wherein the nanoparticles are semiconductive.

5. The method of claim 1 wherein the nanoparticles are conductive.

6. The method of claim 1 wherein the nanoparticles, surrounded by the biological material, collectively act as an insulator.

7. The method of claim 1 wherein the component is a transistor comprising a source element and a drain element and a semiconductor layer disposed between the source and the drain elements, and depositing the nanoparticles onto a surface comprises depositing the nanoparticles onto the surface of the semiconductor layer.

8. The method of claim 1 repeated at a plurality of locations on a substrate to form an array of bioelectronic components.

12. The method of claim 1 wherein the biological material comprises at least one nucleic acid.

13. The method of claim 1 wherein the biological material comprises at least one protein.

9. A method for fabricating a biolectronic component, the method comprising the steps of:

a. providing a first batch of nanoparticles having submicron sizes and a first electrical characteristic;

b. depositing the first batch of nanoparticles onto a surface;

c. sintering the first batch of nanoparticles to form a continuous, uniform layer exhibiting the electrical characteristic of the first batch of nanoparticles, the layer having a surface;

d. providing a second batch of nanoparticles having submicron sizes and a second electrical characteristic;

e. attaching at least one biological material to the second batch of nanoparticles so as to form shells of the shells of the biological material therearound;

f. depositing the second batch of nanoparticles onto the layer surface formed by the first batch of nanoparticles;

g. causing the deposited second batch of nanoparticles to be in electrical communication with at least one electrical contact to facilitate an electrical measurement thereof, the electrical measurement being affected by the biological material.

10. A method for fabricating a bioelectronic component, the method comprising the steps of:

a. providing a first batch of nanoparticles having submicron sizes and a first electrical characteristic;

b. depositing the first batch of nanoparticles onto a surface;

c. sintering the first batch of nanoparticles to form a continuous, uniform layer exhibiting the electrical characteristic of the first batch of nanoparticles, the layer having a surface;

d. providing a second batch of electrically conductive nanoparticles having submicron sizes;

e. depositing the second-batch nanoparticles in contact with a portion of the layer derived from the first batch of nanoparticles; and

f. sintering the second-batch of nanoparticles to form an electrical contact,

g. providing a third batch of nanoparticles having submicron sizes and a second electrical characteristic;

h. attaching at least one biological material to the third batch of nanoparticles so as to form shells of the shells of the biological material therearound;

i. depositing the third batch of nanoparticles onto the layer surface formed by the first batch of nanoparticles;

j. causing the deposited second batch of nanoparticles to be in electrical communication with the electrical contact to facilitate an electrical measurement thereof, the electrical measurement being affected by the biological material.

11. The method of claim 10 further comprising the steps of repeating steps (a)-(j) at a plurality of locations on a substrate to form an array of bioelectronic components.