Microfluidic device with sample injector and method of using
First Claim
1. A method of injecting a liquid sample into an electrolyte channel in a microfluidics device having a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively, where at least one of the ports is axially spaced along the electrolyte channel from the other two ports, said method comprising(a) supplying a sample to the first side channel, (b) applying across the first side channel and at least one of the other two side channels, a voltage potential effective to move sample in the first channel into a volume element of the electrolyte channel extending between the first port and at least one other port which is axially offset from the first port, (c) simultaneously controlling the voltage applied to the three side channels, and optionally, at least one of said upstream and downstream channel end portions, to create a sample volume element in the electrolyte channel that has a desired leading- and trailing-edge shape and/or distribution of sample components within the volume element, and (d) simultaneously controlling the voltage applied to the upstream and downstream channel portion, and to at least two of the side channels, to advance the sample element having a desired leading- and trailing-edge shape and/or distribution of sample components in a downstream direction within the electrolyte channel.
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Abstract
A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.
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Citations
19 Claims
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1. A method of injecting a liquid sample into an electrolyte channel in a microfluidics device having a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively, where at least one of the ports is axially spaced along the electrolyte channel from the other two ports, said method comprising
(a) supplying a sample to the first side channel, (b) applying across the first side channel and at least one of the other two side channels, a voltage potential effective to move sample in the first channel into a volume element of the electrolyte channel extending between the first port and at least one other port which is axially offset from the first port, (c) simultaneously controlling the voltage applied to the three side channels, and optionally, at least one of said upstream and downstream channel end portions, to create a sample volume element in the electrolyte channel that has a desired leading- and trailing-edge shape and/or distribution of sample components within the volume element, and (d) simultaneously controlling the voltage applied to the upstream and downstream channel portion, and to at least two of the side channels, to advance the sample element having a desired leading- and trailing-edge shape and/or distribution of sample components in a downstream direction within the electrolyte channel.
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13. A microfluidic system designed for use in injecting a defined-volume liquid sample into an electrolyte channel, for transport through the channel, comprising
a microfluidic device having a channel network that includes such an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively, where at least one of the ports is axially spaced along the electrolyte channel from the other two ports, ports for supplying liquid medium to the electrolyte channel and the side channels, upstream and downstream electrodes, and first, second, and third electrodes adapted to communicate with liquid medium contained in upstream and downstream portions of the electrolyte channel, and the first, second, and third side channels, respectively, and a voltage controller operatively connected to the upstream downstream, and first, second, and third electrodes, which operates to (a) applying across the first side channel and at least one of the other two side channels, a voltage potential effective to move a liquid sample contained in the first channel into a volume element of the electrolyte chamber extending between the first and at least one other port which is axially offset from the first port, (b) simultaneously control the voltage applied to the three side channels, and at least one of said upstream and downstream channel end portions, to create a sample volume element in the electrolyte channel that has a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements, and (c) simultaneously control the voltage applied to the upstream and downstream channel portion, and to at least two of the side channels, to advance the sample element having a desired leading- and trailing-edge shape and/or distribution of sample components in a downstream direction within the electrolyte channel.
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15. The system of 14, wherein controlling step (d) is effective to move an electrolyte solution in the upstream channel portion successively through the second, first and third ports, to move sample contained in the three side channels away from the electrolyte channel.
Specification