Tandem high field asymmetric waveform ion mobility spectrometry (FAIMS)tandem mass spectrometry

• US 6,822,224 B2
• Filed: 09/03/2002
• Issued: 11/23/2004
• Est. Priority Date: 03/14/2000
• Status: Expired due to Fees

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Guevremont, Roger
• Primary Examiner(s): Wells, Nikita
• Assistant Examiner(s): EL SHAMMAA, MARY A
• Application Number:
  US10/220,605
• Publication Number:
  US 20030020012A1
• Time in Patent Office: 812 Days
• Field of Search: 250/281, 250/282, 250/286, 250/287, 250/288, 250/289, 250/292, 250/297, 250/306, 250/307
• US Class Current: 250/287
• CPC Class Codes: G01N 27/624 Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]
• CPC Class Codes: H01J 49/004 Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn

Abstract

A tandem mass spectrometer is disclosed. A first mass analyzer within a low-pressure region is provided for passing ions therethrough. A collision cell is at an ion outlet of the mass analyzer to provide a location for ions to collide therein with a collision gas to form resultant ions. The resultant ions are then provided to a FAIMS analyzer for separation thereof and the separate resultant ions are provided to a mass analyzer for analysis.

Claims

1. A tandem mass spectrometer comprising a first mass analyzer within a low pressure region, a collision cell and a second mass analyzer within the low pressure region, characterized in that between the collision cell and the second mass analyzer is disposed a FAIMS analyzer.

2. An apparatus according to claim 1, wherein the collision cell is disposed within a chamber for containing a collision gas.

3. An apparatus according to claim 1, wherein the FAIMS analyzer comprises:

first and second spaced apart electrodes defining a FAIMS analyzer region therebetween, the FAIMS analyzer region having a first ion inlet and a first ion outlet, the first ion inlet for, in use, receiving ions for introduction into the FAIMS analyzer region, the first ion outlet for, in use, providing ions from the FAIMS analyzer region.

4. An apparatus according to claim 3, wherein the FAIMS analyzer comprises:

at least a first voltage source for providing an asymmetric waveform and a direct-current compensation voltage to at least one of the first and second spaced apart electrodes, to form a first electric field therebetween, the asymmetric waveform for, in use, effecting a difference in net displacement between the ions in the time of one cycle of the applied asymmetric waveform and the compensation voltage for, in use, effecting a first separation of the ions supporting selective transmission of the ions within the FAIMS analyzer region.

5. An apparatus according to claim 4, wherein the low pressure region is a low pressure region within a vacuum chamber.

6. An apparatus according to claim 5, comprising a second chamber within the vacuum chamber, the second chamber having a second ion inlet, a second ion outlet and a gas inlet, the second ion inlet for, in use, introducing ions into second chamber and out of the second ion outlet, the gas inlet for introducing at least a gas having a predetermined composition into the second chamber to define at least a high pressure region.

7. An apparatus according to claim 6, wherein the collision cell and the FAIMS analyzer are disposed within the second chamber, the collision cell in fluid communication with the second ion inlet.

8. An apparatus according to claim 7, comprising a partition for segmenting the second chamber into at least two compartments, the at least two compartments in fluid communication one with the other and wherein the collision cell and the FAIMS analyzer are each disposed within a different compartment of the second chamber.

9. An apparatus according to claim 6, wherein the collision cell is disposed within the second chamber and comprising a third chamber including an ion inlet, an ion outlet and housing the FAIMS, the third chamber in fluid communication with the second chamber.

10. An apparatus according to claim 3, wherein the FAIMS analyzer comprises:

a first voltage source for providing a voltage having an approximately sinusoidally varying amplitude to at least one of the first and second spaced apart electrodes of the FAIMS, to form a first electric field therebetween, the first electric field for transmitting ions through the FAIMS analyzer such that ions are other than separated by FAIMS.

11. An apparatus according to claim 10, wherein the collision cell and the FAIMS analyzer are disposed within separate chambers for containing a pressure, such that the FAIMS analyzer is operable in a first mode under low pressure and in a second other mode under higher pressure independent of the pressure within the chamber housing the collision cell.

12. An apparatus according to claim 11, wherein the FAIMS analyzer comprises third and fourth electrodes, arranged such that the four electrodes of the FAIMS analyzer are disposed along corner edges of a quadrilateral solid.

13. A tandem mass spectrometer comprising a first mass analyzer within a low pressure region;

a FAIMS for providing a collision cell and ion separation; and

a second mass analyzer within a low pressure region.

14. An apparatus according to claim 13, wherein the FAIMS analyzer comprises:

first and second spaced apart electrodes defining a FAIMS analyzer region therebetween, the FAIMS analyzer region having a first ion inlet and a first ion outlet, the first ion inlet for, in use, receiving ions for introduction into the FAIMS analyzer region, the first ion outlet for, in use, providing ions from the FAIMS analyzer region.

15. An apparatus according to claim 14, wherein the FAIMS analyzer comprises:

at least a first voltage source for providing an asymmetric waveform and a direct-current compensation voltage to at least one of the first and second spaced apart electrodes, to form a first electric field therebetween, the asymmetric waveform for, in use, effecting a difference in net displacement between the ions in the time of one cycle of the applied asymmetric waveform and the compensation voltage for, in use, effecting a first separation of the ions supporting selective transmission of the ions within the FAIMS analyzer region.

16. An apparatus according to claim 15, wherein the FAIMS analyzer is disposed within a chamber for containing a pressure, such that the FAIMS analyzer is operable in a first mode under low pressure and in a second other mode under higher pressure.

17. An apparatus according to claim 16, wherein the FAIMS analyzer comprises third and fourth electrodes, arranged such that the four electrodes of the FAIMS analyzer are disposed along corner edges of a quadrilateral solid.

18. The apparatus according to claim 13, wherein the FAIMS analyzer comprises:

four electrodes disposed approximately along corner edges of a quadralateral solid for transmitting ions through a low pressure region in a first mode of operation and for providing a field for effecting a separation of ions in a second other mode of operation.

19. An apparatus for separating ions comprising:

a) a FAIMS analyzer region defined by a space between first and second spaced apart electrodes;

b) a collision region in operational communication with the FAIMS analyzer region for providing ions to the FAIMS analyzer region, the collision region defined by a space between two electrodes, the collision region having a first gas inlet, the first gas inlet for providing a flow of a collision gas within the collision region;

c) an ion source for providing ions to the collision region; and

,

d) a voltage source for providing at least a voltage to at least one of the first and second electrodes of the FAIMS analyzer region, to form an electric field therebetween, the electric field for effecting a separation of the provided ions having an approximately same mass-to-charge ratio,

wherein the ions provided to the collision region interact with the collision gas to produce collisionally induced fragment ions, and wherein the ions provided to the FAIMS analyzer region include the collisionally induced fragment ions.

20. A method for separating ions comprising:

providing ions to a first mass analyzer for transmission therethrough to a collision region having a collision gas therein;

colliding the ions with the collision gas to produce a plurality of resultant ions;

transporting the resultant ions through an electric field resulting from application of an asymmetric waveform to an electrode to perform a separation thereof; and

,

providing some of the separated ions to a second mass analyzer for analysis.

21. A method for separating ions according to claim 20, wherein the resultant ions are collisionally induced fragment ions.

22. A method according to claim 20, wherein electric field is formed by:

i) providing a first asymmetric waveform and a first direct-current compensation voltage, to at least one electrode, to form an electric field therebetween, the first asymmetric waveform for effecting a difference in net displacement between two different ions in the time of one cycle of the applied first asymmetric waveform; and

,

ii) setting the first compensation voltage for effecting a separation of the fragment ions having an approximately same mass-to-charge ratio, to support selective transmission of the ions within the first analyzer region.

23. A method according to claim 20, including detecting the selectively transmitted ions by mass spectrometry.

24. A method according to claim 23, wherein the collisions and the electric field are disposed within at least a high-pressure region.

25. A method according to claim 24, wherein the at least a high-pressure region is disposed within a chamber within a low-pressure region.

26. A method according to claim 25, wherein the low pressure region is a low-pressure region of a vacuum chamber.

27. A method according to claim 22, wherein the electrodes are shaped for focusing the ions in a predetermined fashion.

28. A method according to claim 27, wherein the electrodes are selected from the group including:

flat parallel plates;

curved plates;

cylinders; and

, parallel rods.

29. A method according to claim 28, wherein the voltage applied to the electrode has an amplitude varying in a sinusoidal fashion over time for operating the first analyzer region in an RF-only mode in which ions are other than separated by FAIMS.

30. The method according to claim 28, wherein the electric field is provided between at least two pairs of electrodes and wherein the voltage applied to the electrode has an amplitude varying in a sinusoidal fashion over time and a dc voltage.