Molecular recognition type chemical CCD

• US 6,824,660 B2
• Filed: 12/29/2000
• Issued: 11/30/2004
• Est. Priority Date: 02/29/2000
• Status: Expired due to Fees

• Current Assignee: Horiba Limited
• Original Assignee: Horiba Limited
• Inventors: Tomita, Katsuhiko
• Primary Examiner(s): Bell, Mark L.
• Assistant Examiner(s): Brown, Jennine M.
• Application Number:
  US09/750,357
• Publication Number:
  US 20010017265A1
• Time in Patent Office: 1,432 Days
• Field of Search: 204/416, 435/291, 435/6, 324/457, 257/253
• US Class Current: 204/416
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 27/4473 by electric means
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

A molecular recognition layer 21 is formed on a sensor face 6a of a chemical CCD 1 having such a structure that a plurality of potential wells 6 constituted to change a depth corresponding to a chemical quantity are arranged two-dimensionally, electric charges are injected into the potential wells 6, and the chemical quantity is converted into an electric charge corresponding to the sizes of the potential wells. Thus, it is provided a novel and useful molecular recognition type chemical CCD capable of measuring an ultramicro chemical substance with an ultrahigh sensitivity at a molecular level.

Claims

1. A molecular recognition type chemical CCD comprising:

a chemical CCD having a plurality of potential wells constituted to change a depth corresponding to a chemical quantity, and being arranged two-dimensionally, in which electric charges are injected into the potential wells and the chemical quantity is converted into an electric charge corresponding to the sizes of the potential wells;

a molecular recognition layer formed on a sensor face of a chemical CCD,

said molecular recognition layer being structured to selectively capture molecular of certain chemical substances to allow the chemical CCD to output signals corresponding to the captured molecules.

2. The molecular recognition type chemical CCD according to claim 1, wherein the molecular recognition layer is formed by a molecule imprinting method in which a host polymeric resin and a guest substance to be measured are complexed and polymerized, and said guest substance is then removed to form a mold having a cavity in a portion of the host polymeric resin where the guest substance was present.

3. The molecular recognition type chemical CCD according to claim 1, wherein the molecular recognition layer comprises a DNA complementary to a DNA to be measured.

4. The molecular recognition type chemical CCD according to any of claims 1 to 3, wherein a gel having a solution containing a substance to be measured mixed into a gel material for migration is provided on an upper surface of the molecular recognition layer and a DC voltage is applied to the gel to measure the substance to be measured in the gel which is obtained after electrophoresis.