Protein and peptide sensors using electrical detection methods
First Claim
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1. A method of electrically detecting a molecular interaction between an immobilized probe molecule and a protein or peptide target molecule, comprising:
- (a) detecting a first electrical signal in one or a plurality of microelectrodes in contact with linker moieties to which probe molecules are immobilized, (b) exposing said one or a plurality of microelectrodes to a sample mixture containing a protein or peptide target molecule, (c) detecting a second electrical signal in said one or a plurality of microelectrodes, (d) comparing said first electrical signal with said second electrical signal, (e) determining whether said first electrical signal is different from said second electrical signal, (f) exposing said one or a plurality of microelectrodes to one or a plurality of target binding molecules comprising an electrochemical label, (g) detecting a third electrical signal in said one or a plurality of microelectrodes, and (h) comparing said second electrical signal with said third electrical signal.
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Abstract
The present invention provides an apparatus and methods for the electrical detection of molecular interactions between a probe molecule and a protein or peptide target molecule, but without requiring the use of electrochemical or other reporters to obtain measurable signals. The methods can be used for electrical detection of molecular interactions between probe molecules bound to defined regions of an array and protein or peptide target molecules which are permitted to interact with the probe molecules.
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Citations
20 Claims
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1. A method of electrically detecting a molecular interaction between an immobilized probe molecule and a protein or peptide target molecule, comprising:
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(a) detecting a first electrical signal in one or a plurality of microelectrodes in contact with linker moieties to which probe molecules are immobilized, (b) exposing said one or a plurality of microelectrodes to a sample mixture containing a protein or peptide target molecule, (c) detecting a second electrical signal in said one or a plurality of microelectrodes, (d) comparing said first electrical signal with said second electrical signal, (e) determining whether said first electrical signal is different from said second electrical signal, (f) exposing said one or a plurality of microelectrodes to one or a plurality of target binding molecules comprising an electrochemical label, (g) detecting a third electrical signal in said one or a plurality of microelectrodes, and (h) comparing said second electrical signal with said third electrical signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification