Regulation of endogenous gene expression in cells using zinc finger proteins
First Claim
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1. A method of inhibiting expression of an endogenous cellular gene in a cell, the method comprising the step of:
- contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered and wherein the Kd of the zinc finger protein is less than about 25 nM;
thereby inhibiting expression of the endogenous cellular gene.
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Abstract
The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
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Citations
66 Claims
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1. A method of inhibiting expression of an endogenous cellular gene in a cell, the method comprising the step of:
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contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered and wherein the Kd of the zinc finger protein is less than about 25 nM;
thereby inhibiting expression of the endogenous cellular gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of inhibiting expression of an endogenous cellular gene in a cell, the method comprising the step of:
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contacting a target site in the endogenous cellular gene with an engine fusion zinc finger protein comprising six fingers and a regulatory domain, wherein the Kd of the zinc finger protein is less than about 25 nM;
thereby inhibiting expression of the endogenous cellular gene.
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24. A method of activating expression of an endogenous cellular gene, the method comprising the step of:
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contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered and wherein the Kd of the zinc finger protein is less than about 25 nM;
thereby activating expression of the endogenous cellular gene. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. A method of activating expression of an endogenous cellular gene, the method comprising the step of:
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contacting a target site in the endogenous cellular gene with an engineered fusion zinc finger protein comprising six fingers and a regulatory domain, wherein the Kd of the zinc finger protein is less than about 25 nM;
thereby activating expression of the endogenous cellular gene.
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46. A method of modulating expression of an endogenous cellular gene in a cell, the method comprising the step of:
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contacting a first target site in the endogenous cellular gene with a first zinc finger protein, wherein said first zinc finger protein is engineered;
thereby modulating expression of the endogenous cellular gene. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65)
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66. A method of modulating expression of an endogenous cellular gene in a cell, the method comprising the step of:
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contacting a target site in the endogenous cellular gene with an engineered fusion zinc finger protein comprising six fingers and a regulatory domain;
thereby modulating expression of the endogenous cellular gene.
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Specification