Spectral volume microprobe arrays

• US 6,826,422 B1
• Filed: 01/11/2000
• Issued: 11/30/2004
• Est. Priority Date: 01/13/1997
• Status: Expired due to Fees

• Current Assignee: Luma Imaging Corp. (SpectraScience, Inc.)
• Original Assignee: MediSpectra, Inc. (SpectraScience, Inc.)
• Inventors: Kwo, Jennie, Lipson, David, Bee, David, Emans, Matthew, Hed, Zeʼev, Modell, Mark, Nordstrom, Robert
• Primary Examiner(s): Smith, Ruth S.
• Application Number:
  US09/481,762
• Time in Patent Office: 1,785 Days
• Field of Search: 600/121-125, 600/310, 600/407, 600/473, 600/475-479, 356/317, 356/318, 356/337, 356/341-343, 250/341.1, 250/363-1-3, 250/372, 250/458.1, 250/459.1, 250/461.1, 250/461.2
• US Class Current: 600/407
• CPC Class Codes: A61B 1/00059 provided with identification means for the endoscope
• CPC Class Codes: A61B 1/00142 with means for preventing contamination, e.g. by using a sanitary sheath
• CPC Class Codes: A61B 2090/0814 Preventing re-use
• CPC Class Codes: A61B 2562/08 Sensors provided with means for identification, e.g. barcodes or memory chips
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

Methods and apparatus are provided for determining a characteristic of a sample of a material by the interaction of electromagnetic radiation with the sample. The apparatus includes an optical assembly and a protective barrier. The optical assembly sequentially illuminates a plurality of volume elements in the sample with an intensity distribution in the sample that drops off substantially monotonically from a first region in a first optical path and collects electromagnetic radiation emanating from each of the volume elements. The optical assembly collects the electromagnetic radiation emanating from each of the volume elements with a collected distribution that drops off substantially monotonically from a second region in a second optical path. The first and second regions at least partially overlap in each of the volume elements. The optical assembly can be configured as a probe, to be directed to the evaluation of a sample of a biological material. A protective barrier can be disposed between the optical assembly and a body tissue, to prevent contamination of the optical assembly by said body tissue.

Claims

1. Apparatus for determining a characteristic of a sample of biological material, comprising:

a probe having an optical assembly that sequentially directs a first set of electromagnetic radiation to a plurality of locations in a sample with an intensity distribution in the sample that drops off substantially monotonically from a first region in a first optical path and that receives a response indicative of a second set of electromagnetic radiation, said second set comprising electromagnetic radiation emanating from each of said locations, said optical assembly collecting said second set of electromagnetic radiation with a collection distribution that drops off substantially monotonically from a second region in a second optical path, said first and second regions at least partially overlapping in each of said locations, said optical assembly comprising at least one array of field stops whose dimensions are large compared to a quotient of wavelength of said electromagnetic radiation divided by a working numerical aperture of said optical assembly, measured from said field stops;

a detector coupled to said received response to produce signals that vary according to said characteristic in each of said locations;

a processor that processes signals produced by the detector to determine the characteristic of the sample; and

a sheath that covers the probe.

2. The apparatus of claim 1 wherein the probe comprises at least one pin and the sheath comprises at least one region corresponding to the at least one pin of the probe.

3. apparatus according to claim 2, wherein the optical assembly is inoperative in the absence of the sheath.

4. The apparatus according to claim 2, wherein the sample of biological material exists in continuity with an in-vivo body tissue.

5. The apparatus according to claim 4, wherein the sheath prevents the probe from contacting the in-vivo body tissue.

6. The apparatus according to claim 4, wherein the sheath prevents the probe from contacting a tissue in proximity to the in-vivo body tissue.

7. The apparatus according to claim 4, wherein the sheath is for a single use.

8. The apparatus according to claim 7, wherein the sheath is for positioning upon the probe in a unique position.

9. The apparatus according to claim 7, wherein the sheath is attached to the probe, and wherein a detachment of the sheath from the probe renders the sheath inoperable.

10. The apparatus according to claim 7, wherein the sheath further comprises a marker bearing data identifying the sheath, and wherein the probe further comprises a reader to read the data on the marker, and a processing system to correlate the data on the marker with an indicator that relates to an unused state of the sheath.

11. The apparatus according to claim 7, wherein the sheath further comprises a marker bearing data identifying the sheath, and wherein the probe further comprises a reader to read the data on the marker, and an indicator that relates to a previously used state of the sheath.

12. The apparatus according to claim 7, further comprising a refractive index matching fluid.

13. The apparatus according to claim 2, wherein the sheath comprises a window for transmitting electromagnetic radiation.

14. The apparatus according to claim 13, wherein the window is capable of transmitting electromagnetic radiation without producing significant fluorescent response.