Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices
First Claim
1. A method of producing illuminating radiation for fluorescence and reflectance imaging, the method comprising selectively producing first and second spectral distributions of electromagnetic radiation for fluorescence/near infrared (NIR) reflectance imaging and white light reflectance imaging respectively, said first spectral distribution comprising an excitation component received from a first optical subsystem of an optical system and a NIR component received from a second optical subsystem of said optical system, said second spectral distribution comprising a white light illumination component received from said first optical subsystem.
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Abstract
Methods and apparatus for contemporaneous measurements of electromagnetic radiation with multiple measuring devices, for producing a high diagnostic sensitivity image while achieving high diagnostic specificity with spectroscopy, for producing illumination for fluorescence/NIR reflectance imaging and white light reflectance imaging, all with the same sensors are disclosed. The method may involve selectively adjusting a gain of an imaging device in at least one wavelength band relative to a gain in at least one other band to produce an optimized image of an object, and may also involve producing a first reflectance signal in a first NIR wavelength band, and producing a second reflectance signal in a second NIR band such that an absorption coefficient ratio of oxyhemoglobin to deoxyhemoglobin in the second wavelength band differs from that in the first wavelength band, to permit the first and second reflectance signals to be used to produce a tissue oxygenation image.
135 Citations
17 Claims
- 1. A method of producing illuminating radiation for fluorescence and reflectance imaging, the method comprising selectively producing first and second spectral distributions of electromagnetic radiation for fluorescence/near infrared (NIR) reflectance imaging and white light reflectance imaging respectively, said first spectral distribution comprising an excitation component received from a first optical subsystem of an optical system and a NIR component received from a second optical subsystem of said optical system, said second spectral distribution comprising a white light illumination component received from said first optical subsystem.
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11. A method of producing a fluorescence image of tissue, the method comprising:
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a) producing ratio signals such that for each point in said tissue, a strength of said ratio signal corresponding to said point is proportional to a ratio of an intensity of reflectance of said point in a first near infrared (NIR) wavelength band to an intensity of fluorescence of said point; and
b) causing said ratio signals to be provided to an input of a display device to produce said fluorescence image of said tissue. - View Dependent Claims (12, 13, 14, 15)
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16. A method of producing a fluorescence image of tissue, the method comprising:
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a) causing fluorescence signals produced in response to fluorescence of said tissue to be provided to a first color channel input of a multicolor display device;
b) causing first near infrared (NIR) reflectance signals produced in response to reflectance of said tissue in a first NIR wavelength band to be provided to a second color channel input of said display device; and
c) causing second NIR reflectance signals produced in response to reflectance of said tissue in a second NIR wavelength band to be provided to a third color channel input of said display device. - View Dependent Claims (17)
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Specification