Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
First Claim
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1. A method for the isolation of stem cells of a mammal, the method comprising:
- obtaining a sample of cells from the mammal;
sorting, from the sample, cells that express β
2-microglobulin from cells that do not express β
2-microglobulin; and
selecting by a stem cell surface marker stem cells from the sample of cells that do not express β
2-microglobulin.
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Abstract
Disclosed herein is the discovery that mammalian stem cells do not express β2microglobulin (β2m). The invention discloses a method of isolating stem cells comprising sorting, from a sample of cells, cells that express β2m from cells that do not express β2m. One then selects stem cells from the population of cells that does not express β2m. This is accomplished by selecting cells that express a known marker, such as proteins expressed by genes encoding the major histocompatibility complex. An isolated stem cell is disclosed, as is a method for identifying it and other stem cells.
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34 Claims
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1. A method for the isolation of stem cells of a mammal, the method comprising:
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obtaining a sample of cells from the mammal;
sorting, from the sample, cells that express β
2-microglobulin from cells that do not express β
2-microglobulin; and
selecting by a stem cell surface marker stem cells from the sample of cells that do not express β
2-microglobulin.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for the isolation of stem cells of a mammal, the method comprising:
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obtaining a sample of cells from the mammal;
sorting, from the sample, cells that express β
2-microglobulin from cells that do not express β
2-microglobulin; and
sorting, from the sample of cells that do not express β
2-microglobulin, cells that express a stem cell surface marker from cells that do not express a stem cell surface marker.- View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification