Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism
First Claim
1. A method for the production of an increased level of very long chain fatty acid molecules in a plant seed cell, said method comprising the steps of:
- growing a plant under conditions wherein said plant produces long chain fatty acyl-CoA molecules in said plant seed cell, in the presence of an expression product of a jojoba β
-ketoacyl-CoA synthase DNA sequence operably linked to regulatory elements for directing the expression of said DNA sequence such as to effect the contact between such long chain fatty acyl-CoA molecules and said expression product, and producing said very long chain fatty acid molecules in said plant seed cell at a level that is increased relative to the native level of said very long chain fatty acid molecules in said plant seed cell.
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Accused Products
Abstract
By this invention, a plant β-ketoacyl-CoA synthase condensing enzyme is provided free from intact cells of said plant and capable of catalyzing the production of very long chain fatty acid molecules. Also contemplated are constructs comprising the nucleic acid sequence and a heterologous DNA sequence not naturally associated with the condensing enzyme encoding sequences, and which provide for at least transcription of a plant condensing enzyme encoding sequence in a host cell. In this fashion very long chain fatty acid molecules may be produced in a plant cell. Included are methods of modifying the composition of very long chain fatty acid molecules in a plant cell.
19 Citations
16 Claims
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1. A method for the production of an increased level of very long chain fatty acid molecules in a plant seed cell, said method comprising the steps of:
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growing a plant under conditions wherein said plant produces long chain fatty acyl-CoA molecules in said plant seed cell, in the presence of an expression product of a jojoba β
-ketoacyl-CoA synthase DNA sequence operably linked to regulatory elements for directing the expression of said DNA sequence such as to effect the contact between such long chain fatty acyl-CoA molecules and said expression product,and producing said very long chain fatty acid molecules in said plant seed cell at a level that is increased relative to the native level of said very long chain fatty acid molecules in said plant seed cell. - View Dependent Claims (2, 3, 4, 5, 7, 8, 9, 10)
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6. A method for altering the composition of fatty acids in a plant cell, said method comprising the steps of:
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growing a plant under conditions wherein said plant produces long chain fatty acyl-CoA molecules, in the presence of an expression product of a jojoba β
-ketoacyl-CoA synthase DNA sequence operably linked to regulatory elements for directing the expression of said DNA sequence such as to effect the contact between such long chain fatty acyl-CoA molecules and said jojoba β
-ketoacyl-CoA synthase, wherein(i) said jojoba β
-ketoacyl-CoA synthase is capable of catalyzing the production of very long chain fatty acids from a long chain fatty acyl-CoA substrate and malonyl-CoA,(ii) said DNA sequence is heterologous to said plant, and (iii) very long chain fatty acids are produced in said plant such as to alter the overall fatty acid composition of said plant cell. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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Specification
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- Resources
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Current AssigneeMonsanto Company (Bayer AG)
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Original AssigneeCalgene Incorporated (Pharmacia Corp.)
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InventorsLassner, Michael W., Metz, James George, Lardizabal, Kathryn Dennis
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Primary Examiner(s)Fox, David T.
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Assistant Examiner(s)Kallis, Russell
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Application NumberUS08/657,749Time in Patent Office3,113 DaysField of Search800/278, 800/281, 800/287, 800/298, 800/205, 800/250, 435/69.1, 435/69.2, 435/419, 530/350, 530/370, 536/23.2, 536/23.6, 536/24.5US Class Current800/281CPC Class CodesC12N 15/8247 involving modified lipid me...C12N 9/1029 transferring groups other t...
