N,N-dinitramide salts as solubilizing agents for biologically active agents

• US 6,833,478 B2
• Filed: 07/13/2001
• Issued: 12/21/2004
• Est. Priority Date: 07/13/2001
• Status: Expired due to Fees

• Current Assignee: SRI International, Inc.
• Original Assignee: SRI International, Inc.
• Inventors: Bottaro, Jeffrey C., Petrie, Mark A., Penwell, Paul E., Bomberger, David C.
• Primary Examiner(s): DAVIS, BRIAN J
• Application Number:
  US09/905,577
• Publication Number:
  US 20030026850A1
• Time in Patent Office: 1,257 Days
• Field of Search: 514/610, 564/107, 564/109
• US Class Current: 564/107
• CPC Class Codes: A01N 25/02 containing liquids as carriers, diluents or solvents
• CPC Class Codes: A61K 47/02 Inorganic compounds
• CPC Class Codes: A61K 47/52 the modifying agent being an inorganic compound, e.g. an inorganic ion that is complexed with the active ingredient
• CPC Class Codes: C07C 211/32 containing dibenzocycloheptane or dibenzocycloheptene ring systems or condensed derivatives thereof
• CPC Class Codes: C07C 2603/32 Dibenzocycloheptenes; Hydrogenated dibenzocycloheptenes
• CPC Class Codes: C07D 453/04 having a quinolyl-4, a substituted quinolyl-4 or a alkylenedioxy-quinolyl-4 radical linked through only one carbon atom, attached in position 2, e.g. quinine

Abstract

A method is provided for enhancing the solubility of an ionizable compound in a lipophilic medium by admixing the compound with an effective solubility-enhancing amount of an N,N-dinitramide salt. The ionizable compound, upon ionization, gives rise to a biologically active cationic species that ionically associates with the N,N-dinitramide anion N(NO₂)₂⁻ following admixture with the N,N-dinitramide salt. The biologically active cationic species may be a pharmacologically active cation, in which case the method is useful for enhancing the penetration of the blood-brain barrier by the pharmacologically active cation. In other embodiments, the ionizable compounds are medical imaging or diagnostic agents, or agricultural agents such as pesticides. Salts of biologically active cations and N,N-dinitramide ion are also provided as novel compositions of matter.

Claims

1. A method for increasing the solubility of an ionizable compound in a lipophilic medium, wherein ionization of the compound results in a biologically active cationic species in association with an anionic counterion, the method comprising admixing the ionizable compound with an effective solubility enhancing amount of an N,N-dinitramide salt.

2. The method of claim 1, wherein the ionizable compound is a salt comprised of the biologically active cationic species and an anionic counterion.

3. The method of claim 2, wherein the biologically cationic species is a nitrogen-containing cation containing at least one positively charged nitrogen atom.

4. The method of claim 3, wherein the admixing is carried out under conditions that result in replacement of the anionic counterion with N,N-dinitramide anion.

5. The method of claim 1, wherein the ionizable compound is in electronically neutral form prior to admixture with the N,N-dinitramide salt, but upon admixture with the N,N-dinitramide salt ionizes to form a biologically active cationic species ionically associated with N,N-dinitramide anion.

6. The method of claim 1, wherein the ionizable compound becomes protonated in an aqueous medium at physiological pH to give a biologically active cationic species in association with hydroxide counterions.

7. The method of claim 6, wherein the ionizable compound is a nitrogen-containing compound containing at least one nitrogen atom that becomes protonated and thus positively charged in an aqueous medium at physiological pH.

8. The method of claim 1, wherein the ionizable compound is comprised of a non-ionizable precursor modified so as to contain an ionizable site, wherein ionization of the ionizable site results in the biologically active cationic species.

9. The method of claim 1, wherein the N,N-dinitramide salt has the formula M^+x[N(NO₂)₂⁻

]_x wherein M is selected so that it is displaced by the biologically active cationic species upon admixture of the N,N-dinitramide salt with the ionizable compound, and x is the cationic charge of M.

10. The method of claim 1, wherein the N,N-dinitramide salt has the formula M^+x[N(NO₂)₂⁻

]_x wherein M is a cation selected from the group consisting of a metal ion and a nitrogen-containing ion, and x is the cationic charge of M.

11. The method of claim 10, wherein M is a mono, di, or trivalent metal cation.

12. The method of claim 11, wherein M is selected from the group consisting of Li, Na, K, Rb, Cs, Ca, Ba, Sr, Mg, Cu, Ag, Au, Zn, Cd, Hg, Al, Sc, Y, Ga, In, lanthanide elements (57-71), Ti, Zr, Hf, Ge, Sn, V, Nb, Ta, Cr, Mo, W, Mn, Tc, Re, Fe, Co, Ni, Ru, Rh, Pd, Os, Ir, and Pt.

13. The method of claim 12, wherein M is a metal cation selected from the group consisting of Li, Na, K, Be, and Mg.

14. The method of claim 10, wherein M is a nitrogen-containing cation.

15. The method of claim 14, wherein the nitrogen-containing cation is an inorganic nitrogen-containing cation.

16. The method of claim 15, wherein the inorganic nitrogen-containing cation is selected from the group consisting of ammonium, hydrazinium, nitronium and nitrosonium.

17. The method of claim 16, wherein the inorganic nitrogen-containing cation is ammonium.

18. The method of claim 14, wherein the nitrogen-containing cation is an organic nitrogen-containing cation.

19. The method of claim 18, wherein the organic nitrogen-containing cation is a cationic derivative of an organic compound having one or more tetravalent nitrogen atoms.

20. The method of claim 19, wherein the organic nitrogen-containing cation contains 1 to 8 carbon atoms.

21. The method of claim 20, wherein the nitrogen-containing cation contains 1 or 2 carbon atoms.

22. The method of claim 20, wherein M has the formula R_kH_mN_n^+q, wherein:

n is an integer in the range of 1 to 8;

k is an integer in the range of 1 to 2+n;

q is an integer in the range of 1 to n;

m is equal to n+2+q−

k; and

each R is independent selected from the group consisting of C₁-C₁₂ hydrocarbyl moieties.

23. The method of claim 22, wherein each R is independently selected from the group consisting of linear and branched lower alkyl groups.

24. The method of claim 23, wherein M is selected from the group consisting of CH₃NH₃⁺, (CH₃)₂NH₂⁺, (CH₃)₃NH⁺, (CH₃)₄N⁺, C₂H₅NH₃⁺, (C₂H₅)₂NH₂⁺, (C₂H₅)₃NH⁺, (C₂H₅)₄ N⁺, (C₂H₅)(CH₃)NH₂⁺, (C₂H₅)(CH₃) ₂NH⁺, (C₂H₅)₂(CH₃)₂N⁺, (C₃H₇)₄N⁺, (C₄H₉)₄N⁺, CH₃N₂H₄⁺, (CH₃)₂N₂H₃⁺, (CH₃)₃N₂H₂⁺, (CH₃)₄N₂H⁺, and (CH₃)₅N₂⁺.

25. The method of claim 18, wherein the organic nitrogen-containing cation is selected from the group consisting of guanidinium, biguanidinium, guanylurea, ethylenediaminium, piperazinediium, monoaminoguanidinium, diaminoguanidinium, triaminoguanidinium, tetrazolium, aminotetrazolium, amino-ammonium-furazan, polyvinylammonium, and dicyandiamidium.

26. The method of claim 1, wherein the ionizable compound is a pharmacologically active agent, and the biologically active cationic species is a pharmacologically active cationic species.

27. The method of claim 26, wherein the pharmacologically active agent is selected from the group consisting of:

sympathomimetic amines;

neuroprotective agents;

neuroactive amino acids;

neuroactive peptides;

neurotransmitters;

muscarinic receptor agonists and antagonists;

anticholinesterases;

neuromuscular blocking agents;

ganglionic stimulating drugs;

agents to treat neurodegenerative disorders;

anti-epileptic agents;

CNS and respiratory stimulants;

anesthetic agents;

analgesic agents;

antiemetic agents;

antihypertensive agents;

cerebral vasodilators;

hypnotic agents and sedatives;

anxiolytics and tranquilizers;

neuroleptic agents;

anti-microbial agents;

alpha adrenergic receptor antagonists; and

appetite suppressants.

28. The method of claim 27, wherein the pharmacologically active agent is a sympathomimetic amine or a pharmaceutically acceptable acid addition salt thereof.

29. The method of claim 28, wherein the sympathomimetic amine is selected from the group consisting of albuterol, amphetamine, benzphetamine, colterol, diethylpropion, dopamine, dobutamine, ephedrine, epinephrine, ethylnorepinephrine, fenfluramine, fenoldapam, hydroxyamphetamine, ibopamine, isoetharine, isoproterenol, mephentermine, metaproterenol, metaraminol, methoxamine, midodrine, norepinephrine, phendimetrazine, phenmetrazine, phentermine, phenylephrine, phenylethylamine, phenylpropanolamine, prenalterol, propylhexedrine, ritodrine, terbutaline, tyramine, pharmaceutically acceptable acid addition salts thereof, and combinations of any of the foregoing.

30. The method of claim 27, wherein the pharmacologically active agent is a neuroprotective agent.

31. The method of claim 30, wherein the neuroprotective agent is a neurotrophic factor.

32. The method of claim 27, wherein the pharmacologically active agent is a neuroactive amino acid.

33. The method of claim 27, wherein the pharmacologically active agent is a neuroactive peptide.

34. The method of claim 27, wherein the pharmacologically active agent is a muscarinic receptor agonist.

35. The method of claim 27, wherein the pharmacologically active agent is a muscarinic receptor agonist.

36. The method of claim 27, wherein the pharmacologically active agent is an anticholinesterase.

37. The method of claim 27, wherein the pharmacologically active agent is a neuromuscular blocking agent.

38. The method of claim 27, wherein the pharmacologically active agent is a ganglionic blocking drug.

39. The method of claim 27, wherein the pharmacologically active agent is an agent to treat a neurodegenerative disorder.

40. The method of claim 39, wherein the neurodegenerative disorder is Alzheimer'"'"'s disease and the pharmacologically active agent is selected from the group consisting of donepezil, physostigmine, tacrine, pharmaceutically acceptable acid addition salts thereof, and combinations of any of the foregoing.

41. The method of claim 39, wherein the neurodegenerative disorder is Huntington'"'"'s disease and the pharmacologically active agent is selected from the group consisting of fluoxetine, carbamazepine, and pharmaceutically acceptable acid addition salts and combinations thereof.

42. The method of claim 39, wherein the neurodegenerative disorder is Parkinson'"'"'s disease and the pharmacologically active agent is selected from the group consisting of amantadine, apomorphine, bromocriptine, levodopa, pergolide, ropinirole, selegiline, trihexyphenidyl, atropine, scopolamine, glycopyrrolate, pharmaceutically acceptable acid addition salts thereof, and combinations of any of the foregoing.

43. The method of claim 39, wherein the neurodegenerative disorder is amyotrophic lateral sclerosis (ALS) and the pharmacologically active agent is selected from the group consisting of baclofen, diazepam, tizanidine, dantrolene, pharmaceutically acceptable acid addition salts thereof, and combinations of any of the foregoing.

44. The method of claim 27, wherein the pharmacologically active agent is an anti-epileptic agent.

45. The method of claim 27, wherein the pharmacologically active agent is a CNS or respiratory stimulant.

46. The method of claim 27, wherein the pharmacologically active agent is an analgesic agent.

47. The method of claim 46, wherein the analgesic agent is selected from the group consisting of alfentanil, buprenorphine, butorphanol, codeine, drocode, fentanyl, hydrocodone, hydromorphone, levorphanol, meperidine, methadone, morphine, nalbuphine, oxycodone, oxymorphone, pentazocine, propoxyphene, sufentanil, tramadol, apazone, etodolac, diphenpyramide, indomethacin, meclofenamate, mefenamic acid, oxaprozin phenylbutazone, piroxicam, tolmetin, pharmaceutically acceptable acid addition salts thereof, and combinations of any of the foregoing.

48. The method of claim 27, wherein the pharmacologically active agent is a cerebral vasodilator.

49. The method of claim 27, wherein the pharmacologically active agent is a neuroleptic agent.

50. The method of claim 49, wherein the neuroleptic agent is an antidepressant drug selected from the group consisting of tricyclic antidepressants, serotonin reuptake inhibitors and atypical antidepressants.

51. The method of claim 1, wherein the biologically active cationic species is a metal cation, and the ionizable compound is a metal-based drug, an imaging agent, a diagnostic agent, or a mineral supplement.

52. The method of claim 51, wherein the ionizable compound is an agriculturally active chemical compound.

53. The method of claim 52, wherein the agriculturally active chemical compound is a pesticide.

54. The method of claim 53, wherein the pesticide is selected from the group consisting of acaricides, avicides, bacteriocides, fungicides, insecticides, larvicides, miticides molluscicides, nematocides, ovicides, predicides, pupicides, and rodenticides.

55. The method of claim 1, wherein the effective solubility enhancing amount of an N,N-dinitramide salt is selected to provide a molar ratio of the N,N-dinitramide salt to the ionizable compound in the range of about 0.5z:

1 to about 5z;

1 wherein z is the charge of the biologically active cationic species.

56. The method of claim 55, wherein the molar ratio of the N,N-dinitramide salt to the ionizable compound is in the range of about 1z:

1 to about 2z;

1.

57. The method of claim 56, wherein the molar ratio of the N,N-dinitramide salt to the ionizable compound is in the range of about 1z:

1 to about 1.5z;

1.

58. A salt of N,N-dinitramide anion and a biologically active cation.

59. The salt of claim 58, wherein the biologically active cation is selected from the group consisting of pharmacologically active cations, positively charged imaging agents, positively charged diagnostic agents, and cationic pesticides.

60. The salt of claim 59, wherein the biologically active cation is a pharmacologically active cation.

61. The salt of claim 59, wherein the pharmacologically active cation is selected from the group consisting of protonated pharmacologically active agents, pharmacologically active quaternary ammonium cations, and metal cations.

62. A pharmaceutical formulation comprising a salt of N,N-dinitramide anion and a pharmacologically active cation in a pharmaceutically acceptable carrier.

63. The formulation of claim 62, wherein the pharmacologically active cation is selected from the group consisting of protonated pharmacologically active agents, pharmacologically active quaternary ammonium cations, and metal cations.

64. The formulation of claim 63, which is in the form of a tablet.

65. The formulation of claim 63, wherein the pharmaceutical carrier is a lipophilic liquid, and the formulation is in liquid form.

66. The formulation of claim 65, wherein the lipophilic liquid is suitable for oral administration.

67. The formulation of claim 65, wherein the lipophilic liquid is suitable for parenteral administration.

68. A pharmaceutical formulation comprising (a) an ionizable compound that upon ionization gives a pharmacologically active cation, (b) an effective solubility enhancing amount of an N,N-dinitramide salt, and (c) a pharmaceutically acceptable carrier.

69. The formulation of claim 68, wherein the pharmacologically active cation is selected from the group consisting of protonated pharmacologically active agents, pharmacologically active quaternary ammonium cations, and metal cations.

70. The formulation of claim 68, which is in the form of a tablet.

71. The formulation of claim 68, wherein the pharmaceutical carrier is a lipophilic liquid, and the formulation is in liquid form.

72. The formulation of claim 71, wherein the lipophilic liquid is suitable for oral administration.

73. The formulation of claim 71, wherein the lipophilic liquid is suitable for parenteral administration.

74. A biologically active agent delivery system comprised of:

(a) an N,N-dinitramide salt having the formula M^+x[N(NO₂)₂⁻

]_x wherein M is a cation selected from the group consisting of a metal ion and a nitrogen-containing ion, and x is the cationic charge of M; and

(b) an ionizable compound, wherein ionization of the compound results in a pharmacologically active cation.

75. The delivery system of claim 74, wherein the N,N-dinitramide salt and ionizable compound are physically segregated.

76. The delivery system of claim 74, wherein the N,N-dinitramide salt and the ionizable compound are contained within a single composition.