Apparatus and method for imaging

• US 6,833,920 B2
• Filed: 01/12/2002
• Issued: 12/21/2004
• Est. Priority Date: 07/11/2000
• Status: Expired due to Term

• Current Assignee: Maven Biotechnologies, LLC
• Original Assignee: Maven Technologies, LLC
• Inventors: Ralin, David, Rassman, William, Lieberman, Robert A., Kempen, Lothar U.
• Primary Examiner(s): Pham, Hoa Q.
• Application Number:
  US10/046,620
• Publication Number:
  US 20020093654A1
• Time in Patent Office: 1,074 Days
• Field of Search: 356/364-369, 356/128, 356/136, 356/445-448, 422/82.08, 422/82, 422/11, 435/6, 435/7.1, 435/288.7, 435/808, 436/517, 436/805, 436/527
• US Class Current: 356/369
• CPC Class Codes: G01N 2021/212 Arrangement with total internal reflection
• CPC Class Codes: G01N 21/211 Ellipsometry optical thickness measurement G01B11/06
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G06F 9/264 Microinstruction selection based on results of processing
• CPC Class Codes: G06F 9/3846 using static prediction, e.g. branch taken strategy
• CPC Class Codes: G06F 9/3861 Recovery, e.g. branch miss-prediction, exception handling error detection or correction G06F11/00
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

Apparatus for acquiring an image of a specimen comprising a cassette having an optical portion holding a specimen array on a TIR surface and being removably matable to a processing portion having a polarized light beam source and a processing polarization-sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide having a bottom surface with first and second gratings located to direct polarized light to the TIR surface and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell integral with the optical portion as well as means for selecting the direction and wavelength of the polarized light.

Claims

1. An apparatus for imaging, comprising:

a light source emitting a polarized light beam;

an optical assembly including a top surface and a bottom surface, wherein (a) the bottom surface includes first and second grating portions positioned such that the light beam passes through the first grating portion, reflects at the top surface to provide an evanescent field adjacent the top surface, and exits the optical assembly through the second grating portion, and (b) the optical assembly accommodates positioning a specimen array within the evanescent field, such that the specimen array causes spatially distributed polarization changes in the cross-section of the light beam; and

a two-dimensional array detector positioned to detect the spatially distributed polarization changes in the light beam to provide an image of the specimen array.

2. The apparatus of claim 1, wherein said first and second gratings comprise Bragg gratings.

3. The apparatus of claim 1, wherein said first and second gratings comprise electrically alterable gratings, said apparatus also including means for altering said first and second gratings in a manner to change the direction of light incident thereto.

4. The apparatus of claim 3, wherein said first and second gratings comprise electrically alterable holograms.

5. The apparatus of claim 3, further comprising a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light at a corresponding angle of incidence.

6. The apparatus of claim 1, further comprising a wavelength filter for selecting the wavelength of light from said light source assembly.

7. The apparatus of claim 6, wherein said wavelength filter comprises a filter wheel located to intercept light from said light source assembly, said apparatus including means for rotating said wheel.

8. The apparatus of claim 6, wherein said wavelength filter comprises a stack filter.

9. The apparatus of claim 6, further comprising a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light of a corresponding wavelength.

10. The apparatus of claim 1, further comprising an arcuate adjusting mechanism for moving said light source assembly about an arcuate path to selected positions therealong to alter the angle of the light beam into the optical assembly.

11. The apparatus of claim 1, further comprising a flow cell secured to said top surface, said flow cell including an inlet port and an outlet port for flowing analyze across said top surface.

12. The apparatus of claim 11, wherein said flow cell includes means for securing a slide thereto.

13. The apparatus of claim 1, further comprising a computer, said computer including means for converting images from said reflected light into analytical data.

14. The apparatus of claim 1, wherein the light beam undergoes total internal reflection at the top surface.

33. The apparatus of claim 3, wherein said wavelength filter comprises a stack filter.

15. An apparatus for imaging, comprising

a light source emitting a polarized light beam;

an optical assembly including a reflective surface adapted to allow placing thereon a specimen array and a surface having grating portions, the optical assembly being placed to cause the light beam to pass through a first grating portion to direct the light beam to the reflective surface where the light beam is reflected, the specimen array being within an evanescent field associated with the reflection at the reflective surface, and the light beam after said reflection passing through a second grating portion; and

a polarization-sensitive imaging detector, said detector detecting the light beam reflected from the reflective surface including the spatially distributed polarization changes caused by the specimen array.

16. The apparatus of claim 15, wherein the light source and the imaging detector are constructed as a first separate assembly defining a processing assembly, and the optical assembly is constructed as a second separate assembly defining a cassette, the cassette being removably coupled to the processing assembly.

17. The apparatus of claim 16, wherein the cassette comprises an upper surface defining the reflective surface on which the specimen array is placed and a lower surface having the first grating portion and the second grating portion, each grating portion positioned so that the light beam from the processing assembly will pass through the first grating portion into the optical assembly and after reflection will pass through the second grating portion prior to exiting the optical assembly.

18. The apparatus of claim 16, wherein the cassette has a mount or frame portion to which the optical assembly is attached and the mount or frame portion has mating portions and the processing assembly has mating receiving portions such that by matingly coupling the mating portions to the mating receiving portions the cassette is removably coupled to the processing assembly.

19. The apparatus of claim 16, further comprising at least one electronically alterable grating and controllable electronics altering means for electronically controlling at least one grating whereby the angle of the light beam exiting from the at least one electronically alterable grating may be varied.

20. The apparatus of claim 16, wherein the light source in the processing assembly may be changed by an arcuate adjusting mechanism to be directed at selected different angles toward the optical assembly to alter the angle of the light beam into the optical assembly.

21. The apparatus of claim 16, wherein said light source and said processing assembly are movable in relation to each other to enable selecting portions of the specimen army to be sequentially imaged.

22. An imaging apparatus, comprising:

an optical portion defined by an optical member or assembly of members;

a first grating portion at a surface of the optical portion through which a light beam is directed into the optical portion, the first grating portion being configured to direct the light beam through the optical portion to a reflective surface thereby providing an evanescent field, such that, when a specimen array is placed within the evanescent field, spatially distributed polarization changes in the cross-section of the light beam result;

a second grating portion at a surface of the optical portion, the second grating portion being configured for the light beam to exit the optical portion through the second grating portion; and

a two-dimensional array detector, said detector resolving the light beam to provide an image based on the spatially distributed polarization changes.

23. The apparatus of claim 22 in which the light beam exits the optical portion after a single reflection at the reflective surface.

24. The apparatus of claim 22, wherein the light beam is polarized or to entering the optical portion.

25. The apparatus of claim 24, wherein the polarized light beam has a first coherent length and the optical portion has a first distance between the first grating portion and the reflective surface and a second distance between the reflective surface and the second grating portion, said first and second distances each being smaller than said first coherent length.

26. An imaging apparatus, comprising:

a light source assembly for directing a polarized light beam having a first coherent length at a reflective surface in a manner to generate an evanescent field at said reflective surface, such that when a specimen array is placed within the evanescent field, spatially distributed polarization changes in the cross-section of the light beam result;

an optical assembly having a bottom surface spaced apart from said reflective surface a distance smaller than said first coherent length, said bottom surface including first and second grating portions, said grating portions being located and configured to direct light from said source to said reflective surface through the first grating portion and to direct light reflected from said reflective surface through said second grating portion; and

a two-dimensional array detector, said detector resolving the light beam to provide an image based on the spatially distributed polarization changes.

28. The apparatus of claim 26, wherein said gratings comprise electrically alterable gratings, said apparatus also including means for altering said gratings in a manner to change the direction of light incident thereto.

29. The apparatus of claim 28, wherein said gratings comprise electrically alterable holograms.

30. The apparatus of claim 28, further comprising a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light at a corresponding angle of incidence.

31. The apparatus of claim 26, further comprising a wavelength filter for selecting the wavelength of light from said light source assembly.

32. The apparatus of claim 31, wherein said wavelength fiber comprises a filter wheel located to intercept light from said light source assembly, said app including means for rotating said wheel.

34. The apparatus of claim 31, further comprising a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light of a corresponding wavelength.

35. The apparatus of claim 26, further comprising an arcuate adjusting mechanism for moving said light source assembly about an arcuate path to selected positions therealong to alter the angle of the light to the optical assembly.

36. The apparatus of claim 26, further comprising a flow cell secured to said reflective surface, said flow cell including an inlet port and an outlet port for flowing analyte across said reflective surface.

37. The apparatus of claim 36, wherein said flow cell includes means for securing a slide thereto.

38. The apparatus of claim 26, further comprising a computer, said computer including means for converting images from said reflected light into analytical data.

27. The apparatus of claim wherein said first and second gratings comprise Bragg gratings.