Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners

• US 6,835,938 B2
• Filed: 07/31/2002
• Issued: 12/28/2004
• Est. Priority Date: 07/31/2002
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Ghosh, Jayati, Sillman, Debra A., Corson, John F.
• Primary Examiner(s): Hannaher, Constantine
• Application Number:
  US10/210,855
• Publication Number:
  US 20040021091A1
• Time in Patent Office: 881 Days
• Field of Search: 250/458.1
• US Class Current: 250/458.1
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/276 with alternation of sample and standard in optical path
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G02B 21/241 Devices for focusing focusing in general G02B7/28
• CPC Class Codes: G02B 21/244 using image analysis techniques

Abstract

A method and system for determining a biopolymer array substrate thickness dependent optimal focus distance for scanning a molecular array by a molecular array scanner are disclosed. Also disclosed are methods of determining the thickness of a biopolymer array substrate using a position sensitive device (PSD) component of a biopolymer array scanner. Further methods include determining the thickness of said biopolymeric array and automatically selecting an optimal focus distance using the determined thickness and a calibration function on thickness versus optimal focus distance. The subject invention finds use in a variety of different applications, including both genomic and proteomic applications.

Claims

1. A method for determining a biopolymer array substrate thickness dependent optimal focus distance of a biopolymer array scanner, the method comprising:

(a) scanning portions of a reference substrate over a range of positions, each position representing a different distance between an illuminated region of the reference substrate and an emitted-light detection component of the scanner, each portion scanned at a different position;

(b) assembling data collected from scanning the reference substrate into a representation of an intensity/position function; and

(c) employing an array substrate thickness dependent focus-distance determination method to determine an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner.

2. The method according to claim 1, wherein said method further comprises determining the thickness of said reference substrate to obtain a substrate thickness value employed in said array substrate thickness dependent focus-distance determination method.

3. The method according to claim 2, wherein the thickness of said reference substrate is determined using a position sensing detector (PSD) of said biopolymer array scanner.

4. The method according to claim 1, wherein said method comprises determining an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner for two or more reference substrates of different thickness.

5. The method according to claim 4, wherein said method further comprises determining a calibration function of optimal focus distance versus substrate thickness from said determined array substrate thickness dependent optimal focus distances or ranges of focus distances for said biopolymer array scanner for two or more reference substrates of different thickness.

6. The method according to claim 5, wherein said calibration function is stored in a non-volatile memory storage element of said scanner to produce a calibrated scanner.

7. A calibration function prepared according to the method of claim 5 encoded by one of:

(a) storing said calibration function in a machine readable medium;

(b) transmitting said calibration function over an electronic communications medium;

(c) displaying said calibration function on display device; and

(d) printing said calibration function in a human readable medium.

8. The method according to claim 1, wherein scanning portions of a reference substrate over a range of positions relative to an emitted-light detection component further includes:

for each position,

scanning a set of rows,

filtering the intensity values scanned for each row, and

averaging the intensity values for the set of rows into an average intensity value for the position.

9. The method according to claim 1, wherein assembling data collected from scanning the reference substrate into a representation of an intensity/position function further includes associating each position with an average intensity value.

10. The method according to claim 1, wherein employing an array substrate thickness dependent focus-distance determination method to determine an optimal thickness dependent focus distance or range of focus distances comprises employing a method chosen from:

(i) a peak-intensity-focus-distance determination method;

(ii) a window-focus-distance determination method; and

(iii) an average peak-intensity-focus-distance determination method.

11. The method according to claim 10, wherein when said array substrate thickness is between about 0.95 and 1.05 mm, said employed array substrate thickness dependent focus-distance determination method is chosen from:

(i) a peak-intensity-focus-distance determination method; and

(ii) a window-focus-distance determination method.

12. The method according to claim 11, wherein said employed array substrate thickness dependent focus-distance determination method is a peak-intensity-focus-distance determination method.

13. The method according to claim 11, wherein said employed array substrate thickness dependent focus-distance determination method is a window-focus-distance determination method.

14. The method according to claim 10, wherein when said array substrate thickness is less than about 0.95 mm or greater than about 1.05 mm, said employed array substrate thickness dependent focus-distance determination method is chosen from:

(i) a peak-intensity-focus distance determination method; and

(ii) an average-peak-intensity-focus-distance determination method.

15. The method according to claim 14, wherein said employed array substrate thickness dependent focus distance determination method is a peak-intensity-focus distance determination method.

16. The method according to claim 14, wherein said employed array substrate thickness dependent focus-distance determination method is an average-peak-intensity-focus-distance determination method.

17. The method according to claim 14, wherein said average peak-intensity-focus-distance determination method comprises:

(a) determining the position of the peak intensity in the intensity/position function;

(b) identifying a left and right threshold position relative to said determined peak intensity position that have an intensity below a threshold value; and

(c) determining the mean of said left and right threshold positions to determine said array substrate dependent optimal focus distance or range of focus distances.

18. A set of computer instructions for carrying out the method of claim 1 encoded by one of:

storing the computer instructions in a machine readable medium;

transmitting the computer instructions over an electronic communications medium; and

printing the computer instructions in a human readable medium.

19. A method of determining a biopolymer array substrate thickness, said method comprising:

(a) positioning said array substrate on a receiving element of a biopolymer array scanner; and

(b) employing a position sensing detector (PSD) of said biopolymer array scanner to determine said biopolymer array substrate thickness.

20. The method according to claim 19, wherein said employing step (b) comprises:

(i) identifying a first position of said receiving element wherein light reflected from a first surface of said array substrate is received at a predetermined location of said PSD;

(ii) identifying a second position of said receiving element wherein light reflected from a second surface of said array is received at said predetermined location of said PSD; and

(iii) determining the difference of said first and second positions to determine said biopolymer array substrate thickness.

34. A kit for use in a biopolymer array optical scanner, said kit comprising:

(a) a computer-readable medium according to claim 19; and

(b) instructions for operating said scanner according to said programming.

35. The kit according to claim 34, wherein said kit further includes at least one reference array.

21. A biopolymer array scanner comprising:

(a) a probe-molecule excitation system;

(b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity;

(c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and

(d) an automated array substrate thickness dependent focus-distance-determination subsystem.

22. The biopolymer array scanner according to claim 21, wherein said automated array substrate thickness dependent focus-distance-determination subsystem is a set of computer instructions for performing a method comprising:

(a) scanning portions of a reference substrate over a range of positions, each position representing a different distance between an illuminated region of the reference substrate and an emitted-light detection component of the scanner, each portion scanned at a different position;

(b) assembling data collected from scanning the reference substrate into a representation of an intensity/position function; and

employing an array substrate thickness dependent focus-distance determination method to determine an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner.

23. A calibrated biopolymer array scanner comprising:

(a) a probe-molecule excitation system;

(b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity;

(c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and

(d) a calibration function that selects an optimal focus distance based on an array substrate thickness.

24. The scanner according to claim 23, wherein said calibration function is a calibration function of optimal focus distance versus substrate thickness.

25. A method of assaying a sample, said method comprising:

(a) contacting said sample with a biopolymeric array of two or more biopolymer ligands immobilized on a surface of a solid support; and

(b) reading said array with a calibrated biopolymer array optical scanner according to claim 23 to obtain a result.

26. The method according to claim 25, wherein said reading step (b) includes:

(i) determining the thickness of said biopolymeric array; and

(ii) automatically selecting an optimal focus distance using said determined thickness and calibration function.

27. The method according to claim 26, wherein said calibration function is a calibration function of optimal focus distance versus substrate thickness.

28. A method of reading a biopolymer array, said method comprising:

(a) determining said biopolymer array'"'"'s substrate thickness;

(b) automatically selecting an optimal focus distance using the determined substrate thickness and a calibration function on substrate thickness versus optimal focus distance; and

(c) optically reading said biopolymer array to obtain a result using said optimal focus distance.

29. The method according to claim 28, wherein said thickness is determined using the PSD based method according to a method comprising:

(a) positioning said array substrate on a receiving element of a biopolymer array scanner; and

(b) employing a position sensing detector (PSD) of said biopolymer array scanner to determine said biopolymer array substrate thickness.

30. The method according to claim 28, wherein said biopolymer array is chosen from a polypeptide array and a nucleic acid array.

31. The method according to claim 28, further comprising transmitting said result from a first location to a second location.

32. The method according to claim 31, where said second location is a remote location.

33. A method comprising receiving data representing said result of a scan obtained by the method of claim 28.