Device for concentrating and purifying macromolecules

• US 6,837,995 B1
• Filed: 02/08/2000
• Issued: 01/04/2005
• Est. Priority Date: 02/15/1999
• Status: Expired due to Term

• Current Assignee: Sartorius Stedim Biotech GmbH (Sartorius AG)
• Original Assignee: Vivascience AG (Sartorius AG)
• Inventors: Vassarotti, Vincenzo
• Primary Examiner(s): Walker, W. L.
• Assistant Examiner(s): MENON, KRISHNAN S
• Application Number:
  US09/913,781
• Time in Patent Office: 1,792 Days
• Field of Search: 210/650, 210/781, 210/380.1, 210/787, 210/360.1, 210/361, 210/477, 210/321.75, 210/321.6, 210/321.84, 422/72, 422/101, 422/102, 436/177, 436/45
• US Class Current: 210/321.84
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01D 63/081 Manufacturing thereof
• CPC Class Codes: B01D 63/087 Single membrane modules
• CPC Class Codes: B01D 63/16 Rotary, reciprocated or vibrated modules
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis

Abstract

A device for concentrating and/or purifying macromolecules in liquids is disclosed wherein the concentration/purification is accomplished by a membrane separating concentration and filtration chambers, the membrane and chambers being compressively held in fluid-tight relationship by sliding engagement with a sleeve-like housing. A method for assembling such a device is also disclosed.

Claims

1. Device for concentrating and/or purifying macromolecules in a liquid by filtration through a membrane comprising

(a) a two-sided filtration insert comprising:

(i) an upper plate having at least one concentration chamber adapted to receive and contain a liquid containing macromolecules to be processed, each of said at least one concentration chamber being provided with at least one first port that is separate from and in fluid communication with said concentration chamber;

(ii) a membrane support plate having at least one filtrate chamber for supporting the permeate side of at least one membrane, said at least one filtrate chamber provided with at least one second port that is separate from and in fluid communication with said filtrate chamber and;

(iii) at least one membrane having a feed side and a permeate side, said membrane being fluid-tight along its periphery and situated over at least one of said first and second ports and separating said at least one concentration chamber and said at least one filtrate chamber; and

(iv) elastic gasket means arranged around at least one of said first and second ports and in contact with at least one side of said at least one membrane; and

(b) a two-part pressure-resistant sleeve with closed end portions, said two-part sleeve being separate from said filtration insert and matingly engaging said upper plate and said membrane support plate of said filtration insert so as to surround said first and second ports, said two-part sleeve being sized so as to exert and maintain sufficient compressive forces to seal said at least one membrane fluid-tight against said concentration chamber and said filtrate chamber wherein said compressive forces arise solely from the engagement of said two-part sleeve with said upper plate and said membrane support plate.

2. The device of claim 1 wherein said gasket means is in the form of a ridge of elastic material and is integral with at least one of said concentration chamber and said filtrate chamber.

3. The device of claim 1 with a plurality of concentration and filtrate chambers in substantial alignment and adapted to receive and process a plurality of liquid samples.

4. The device of claim 3 adapted to matingly engage the receptacles of a micro titer plate.