Analyte detection system
First Claim
1. A particle comprising:
- a) a fluorescent analyte detection dye, the analyte detection dye being capable of being excited by light at a first excitation wavelength and capable of emitting light at a maximum wavelength when excited, and b) two or more than two fluorescent labels in a first combination of relative amounts, the fluorescent labels being capable of being excited by light of a same second excitation wavelength and capable of emitting lights at maximum wavelengths, distinguishable from each other, respectively, wherein the maximum wavelength of the emitted light of the analyte detection dye is different from the first and second maximum wavelengths of the emitted lights of the fluorescent labels by at least 100 nm, and the first and second excitation wavelengths differ by at least 100 nm and one of the excitation wavelengths is greater than about 750 nm.
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Abstract
An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
81 Citations
27 Claims
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1. A particle comprising:
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a) a fluorescent analyte detection dye, the analyte detection dye being capable of being excited by light at a first excitation wavelength and capable of emitting light at a maximum wavelength when excited, and b) two or more than two fluorescent labels in a first combination of relative amounts, the fluorescent labels being capable of being excited by light of a same second excitation wavelength and capable of emitting lights at maximum wavelengths, distinguishable from each other, respectively, wherein the maximum wavelength of the emitted light of the analyte detection dye is different from the first and second maximum wavelengths of the emitted lights of the fluorescent labels by at least 100 nm, and the first and second excitation wavelengths differ by at least 100 nm and one of the excitation wavelengths is greater than about 750 nm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An analyte detection system comprising:
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a) one or more than one particle, each particle comprising a fluorescent analyte detection dye capable of being excited by light at an excitation wavelength and capable of emitting light when excited at a maximum wavelength, and two or more than two fluorescent labels in a combination of relative amounts, wherein each fluorescent label is capable of being excited by light of a same excitation wavelength and capable of emitting light when excited at maximum wavelengths, distinguishable from each other, respectively, and the maximum wavelength of emitted light of each fluorescent analyte detection dye is different from the maximum wavelength of emitted light of each of the fluorescent labels by at least 100 nm, and the excitation wavelength of each analyte detection dye differs by at least 100 nm from the excitation wavelength of each of the fluorescent labels and one of the excitation wavelengths is greater than about 750 nm;
b) means for exciting the fluorescent dye;
c) means for exciting the first and second fluorescent labels;
d) means for detecting the emitted lights; and
e) means for correlating the detected emitted lights with a particular particle under analysis. - View Dependent Claims (19, 20, 22)
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21. An assay system comprising a particle having:
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a) a fluorescent analyte detection dye capable of being excited by light at a first excitation wavelength and capable of emitting light when excited;
b) two or more than two fluorescent labels, each fluorescent label being capable of being excited by light of a same second excitation wavelength and capable of emitting light when excited at maximum wavelengths, distinguishable from each other, respectively;
c) a first receptor; and
d) an analyte, wherein the analyte, first receptor, and the fluorescent analyte detection dye form a fluorescent complex on the particle, and the emitted light of the fluorescent analyte detection dye is different from the wavelengths of emitted lights of each of the fluorescent labels by at least 100 nm, and the first and second excitation wavelengths differ by at least 100 nm and one of the excitation wavelengths is greater than about 750 nm.
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23. A method for detecting an analyte on a particle comprising:
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a) moving one or more than one particle through an examination zone, each particle having a fluorescent analyte detection dye, and two or more than two fluorescent labels;
b) directing an exciting light of a first wavelength at each particle in the examination zone;
c) directing an exciting light of a second wavelength at each particle in the examination zone, wherein the fluorescent analyte detection dye and the fluorescent labels each produce different emitting lights, the emitting lights each having a maximum wavelength, distinguishable from each other, respectively, wherein the maximum wavelength of the emitted light of the fluorescent analyte detection dye differs from the maximum wavelengths of the emitted lights of each of the fluorescent labels by at least 100 nm, and wherein the wavelengths of the first and second exciting lights differ by at least 100 nm and one of the wavelengths of exciting lights is greater than about 750 nm;
d) detecting the emitted light of the first fluorescent analyte detection dye and the emitted light of the first and second fluorescent labels; and
e) correlating the detected emitted lights with the particle under analysis. - View Dependent Claims (24, 25, 26, 27)
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Specification