Particulate compositions for chemical synthesis

• US 6,838,447 B2
• Filed: 03/26/2002
• Issued: 01/04/2005
• Est. Priority Date: 03/26/2001
• Status: Expired due to Fees

• Current Assignee: Linden Technologies Incorporated
• Original Assignee: Linden Technologies Incorporated
• Inventors: Huang, Tai-Nang
• Primary Examiner(s): Wilson, James O.
• Assistant Examiner(s): Krishnan, Ganapathy
• Application Number:
  US10/108,212
• Publication Number:
  US 20020137719A1
• Time in Patent Office: 1,015 Days
• Field of Search: 514/44, 514/47, 514/48, 514/51, 536/23.2, 435/6, 425/9
• US Class Current: 514/44.00R
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00614 Delimitation of the attachment areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Disclosed are compositions that include triboelectrically chargeable nucleotide particles of less than 50 μm diameter and carrier particles. In one example, a substrate is selectively patterned with the compositions, e.g., by transfer from a selectively charged surface. The compositions can be used to synthesize nucleic acid arrays.

Claims

1. A method of providing a developer composition, the method comprising:

providing particles of a nucleotide monomer that is protected at one terminus, the particles being less than 50 μ

m in average diameter; and

combining the particles with a carrier agent to provide a developer composition.

2. The method of claim 1 wherein the nucleotide monomer is activated at the other terminus.

3. The method of claim 1 wherein the providing comprises jet milling the particles.

4. The method of claim 1 wherein the providing comprises isolating particles that are less than 10 μ

m in diameter.

5. The method of claim 1 wherein the carrier agent comprises ferrite carrier beads.

6. The method of claim 1 further comprising, combining the particles with a surface charge control agent.

7. The method of claim 6 wherein the surface charge control agent includes one or more of:

cabosil, fumed silica, a fluorinated polymer, and polystyrene.

8. The method of claim 1 wherein the particles are less than 10 μ

m in average diameter.

14. The method of claim 8 wherein the charging charges the nucleotide particles to between 5 μ

C/g to 50 μ

C/g.

15. The method of claim 8 wherein the composition further comprises (c) a surface charge control agent.

9. A method for transferring nucleotide particles from a surface to a substrate comprising:

providing a composition that comprises;

(a) chargeable particles of less than 50 microns diameter, the particles include a nucleotide compound that has a phosphoramidite group and a protecting group, and (b) chargeable carrier beads;

triboelectrically charging the nucleotide particles;

contacting the charged nucleotide particles to a selectively charged surface; and

transferring the nucleotide particles from the surface to a substrate.

10. The method of claim 9 wherein the selectively charged surface comprises discrete areas that electrostatically repel the charged nucleotide particles and other areas to which the charged nucleotide particles attach.

11. The method of claim 9 wherein the selectively charged surface is cylindrical.

12. The method of claim 9 wherein the selectively charged surface is the surface of a belt.

13. The method of claim 9 wherein the selectively charged surface is a photoreceptor.