Peptide repeat immunogens

• US 6,838,553 B1
• Filed: 10/05/1999
• Issued: 01/04/2005
• Est. Priority Date: 10/05/1999
• Status: Expired due to Fees

• Current Assignee: Academia Sinica (Government of The Republic of China)
• Original Assignee: Academia Sinica (Government of The Republic of China)
• Inventors: Ting, Chun-Jen, Hsu, Chia-Tse, Hwang, Jaulang
• Primary Examiner(s): DEVI, SARVAMANGALA J N
• Application Number:
  US09/412,558
• Time in Patent Office: 1,918 Days
• Field of Search: 536/23.4, 536/23.1, 536/23.51, 536/23.7, 435/69.7, 435/69.4, 435/69.3, 435/69.1, 424/184.1, 424/192.1, 530/825, 530/350, 530/300
• US Class Current: 536/23.4
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 14/21 from Pseudomonadaceae (F)
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 7/23 Luteinising hormone-releasing hormone [LHRH]; Related peptides
• CPC Class Codes: C12N 2710/24122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The invention relates to a polypeptide including (1) a receptor binding domain of a Pseudomonas exotoxin A, and (2) at least two copies of a peptide sequence.

Claims

1. An isolated nucleic acid encoding a polypeptide, wherein the polypeptide comprises (1) a Pseudomonas exotoxin A fragment consisting of the receptor binding domain of the Pseudomonas exotoxin A and (2) at least two copies of an antigenic peptide sequence wherein said copies occur as a single block of peptide repeats without intervening amino acids in between any two copies.

2. An isolated nucleic acid encoding a polypeptide, wherein the polypeptide comprises (1) a Pseudomonas exotoxin A fragment consisting of the receptor binding domain of a Pseudomonas exotoxin A and (2) at least two copies of an antigenic peptide sequence comprising SEQ ID NO:

1, and has no toxicity.

3. An isolated nucleic acid encoding a polypeptide, wherein the polypeptide comprises (1) a Pseudomonas exotoxin A fragment consisting of the receptor binding domain of a Pseudomonas exotoxin A and (2) 10 to 20 copies of an antigenic peptide sequence, and has no toxicity.

4. An isolated nucleic acid encoding a polypeptide, wherein the polypeptide comprises (1) a Pseudomonas exotoxin A fragment consisting of the receptor binding domain of a Pseudomonas exotoxin A and (2) at least two copies of an antigenic peptide sequence in a consecutive series, and has no toxicity.