Multiplexed fluorescent detection in microfluidic devices
First Claim
1. An optical detection and orientation system for irradiating a fluorescent sample in a detection volume of a microchannel, wherein said microchannel is in a solid substrate, said system comprising:
- a movable optical train comprising a source of excitation light and an optical element for directing said light at said detection volume;
a detector for receiving light emanating from said detection volume and transferring said emanating light for analysis; and
a carrier for moving said optical train and excitation light across the surface of said solid substrate comprising said microchannel in response to changes in light emanating from said detection volume.
4 Assignments
0 Petitions
Accused Products
Abstract
An optical detection and orientation device is provided comprising a housing having an excitation light source, an optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focusing lens for focusing the emitted light onto the entry of an optical fiber, which serves as a confocal aperture, and a moveable carrier for accurately moving said housing over a small area in relation to a channel in a microfluidic device. The optical detection and orientation device finds use in detecting fluorophores in the channel during operations involving fluorescent signals.
171 Citations
44 Claims
-
1. An optical detection and orientation system for irradiating a fluorescent sample in a detection volume of a microchannel, wherein said microchannel is in a solid substrate, said system comprising:
-
a movable optical train comprising a source of excitation light and an optical element for directing said light at said detection volume;
a detector for receiving light emanating from said detection volume and transferring said emanating light for analysis; and
a carrier for moving said optical train and excitation light across the surface of said solid substrate comprising said microchannel in response to changes in light emanating from said detection volume. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. An optical detection and orientation system for detecting fluorescence of a sample material in a detection volume of a microchannel wherein said microchannel is in a solid substrate, said system comprising:
-
at least one source of excitation light optically coupled to a movable optical train, said optical train comprising at least one optical element configured to direct said excitation light from said at least one source to said detection volume;
a carrier supporting said optical train, said carrier being adapted to move said optical train relative to said solid substrate;
at least one optical receiver configured to collect light emanating from said detection volume, and to transfer said emanating light to at least one detector; and
a data analyzer configured to analyze said emanating light. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
-
Specification