DNA base sequencing system
First Claim
1. A system for obtaining nucleic acid sequence information comprising:
- a reaction vessel;
a first capillary or groove supplying dATP into the reaction vessel by pressurizing or by a liquid transfer system a second capillary or groove supplying dGTP into the reaction vessel by pressurizing or by a liquid transfer system;
a third capillary or groove supplying dCTP into the reaction vessel by pressurizing or by a liquid transfer system;
a forth capillary or groove supplying dTTP into the reaction vessel by pressurizing or by a liquid transfer system; and
a detector monitoring synthesis of a strand complementary to a template nucleic acid by detecting chemiluminescence which arises from reaction with ATP and luciferin in the presence of luciferase at the reaction vessel the ATP being converted from pyrophosphate produced from the synthesis which uses one of the dATP, the dGTP, the dCTP and the dTTP, wherein the template nucleic acid is set in the reaction vessel.
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Accused Products
Abstract
The present invention provides a DNA base sequencing system having a compact, simple and convenient structure.
In one embodiment of the present invention, a reaction chamber module for pyrosequencing in which a multiple number of reaction vessels (reaction chambers) 10 and reagent-introducing narrow tubes 6 are integrated is formed in a device board 5. Reagents held in reagent reservoirs 1, 2, 3 and 4 mounted separately from this reaction chamber module are introduced into the reaction vessels 10 via reagent-introducing narrow tubes (capillaries) 6. The reagent-introducing narrow tubes (capillaries) 6 at the area of 2 cm from the reaction vessels 10 are structured with narrow capillaries having an inner diameter of about 0.1 mm and the conductance of these capillaries for the reagent solution determines the injection speed of the solution.
Using the present invention, many kinds of DNAs can be analyzed simultaneously.
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Citations
14 Claims
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1. A system for obtaining nucleic acid sequence information comprising:
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a reaction vessel;
a first capillary or groove supplying dATP into the reaction vessel by pressurizing or by a liquid transfer system a second capillary or groove supplying dGTP into the reaction vessel by pressurizing or by a liquid transfer system;
a third capillary or groove supplying dCTP into the reaction vessel by pressurizing or by a liquid transfer system;
a forth capillary or groove supplying dTTP into the reaction vessel by pressurizing or by a liquid transfer system; and
a detector monitoring synthesis of a strand complementary to a template nucleic acid by detecting chemiluminescence which arises from reaction with ATP and luciferin in the presence of luciferase at the reaction vessel the ATP being converted from pyrophosphate produced from the synthesis which uses one of the dATP, the dGTP, the dCTP and the dTTP, wherein the template nucleic acid is set in the reaction vessel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A nucleic acid analyzing system comprising:
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a reaction vessel;
a first capillary or groove supplying dATP into the reaction vessel by pressurizing or by a liquid transfer system;
a second capillary or grove supplying dGTP into the reaction vessel by pressurizing or by a liquid transfer system;
a third capillary or groove supplying dCTP into the reaction vessel by pressurizing or by a liquid transfer system;
a forth capillary or groove supplying dTTP into the reaction vessel by pressurizing or by a liquid transfer system; and
a detector monitoring synthesis of a strand complementary to a template nucleic acid by detecting chemiluminescence which arises from reaction with ATP and luciferin in the presence of luciferase at the reaction vessel the ATP being converted from pyrophosphate produced from the synthesis which uses one of the dATP, the dGTP, the dCTP and the dTTP, wherein the template nucleic acid is set in the reaction vessel. - View Dependent Claims (10, 11, 12, 13, 14)
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Specification