DNA base sequencing system

• US 6,841,128 B2
• Filed: 03/14/2001
• Issued: 01/11/2005
• Est. Priority Date: 03/17/2000
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Zhou, Guohua, Miyahara, Yuji, Kambara, Hideki
• Primary Examiner(s): Barts, Samuel
• Assistant Examiner(s): Lewis, Patrick T.
• Application Number:
  US09/805,240
• Publication Number:
  US 20010024790A1
• Time in Patent Office: 1,399 Days
• Field of Search: 422/52, 422/69, 422/70, 422/100, 422/101, 422/102, 435/6, 435/91.51, 435/91.1, 435/91.2, 204/452, 536/23.1, 536/25.3, 536/25.31, 536/24.3, 536/25.32
• US Class Current: 422/70
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2565/301 Pyrophosphate (PPi)
• CPC Class Codes: C12Q 2565/537 characterised by the capture oligonucleotide acting as a primer

Abstract

The present invention provides a DNA base sequencing system having a compact, simple and convenient structure.

In one embodiment of the present invention, a reaction chamber module for pyrosequencing in which a multiple number of reaction vessels (reaction chambers) 10 and reagent-introducing narrow tubes 6 are integrated is formed in a device board 5. Reagents held in reagent reservoirs 1, 2, 3 and 4 mounted separately from this reaction chamber module are introduced into the reaction vessels 10 via reagent-introducing narrow tubes (capillaries) 6. The reagent-introducing narrow tubes (capillaries) 6 at the area of 2 cm from the reaction vessels 10 are structured with narrow capillaries having an inner diameter of about 0.1 mm and the conductance of these capillaries for the reagent solution determines the injection speed of the solution.

Using the present invention, many kinds of DNAs can be analyzed simultaneously.

Claims

1. A system for obtaining nucleic acid sequence information comprising:

a reaction vessel;

a first capillary or groove supplying dATP into the reaction vessel by pressurizing or by a liquid transfer system

a second capillary or groove supplying dGTP into the reaction vessel by pressurizing or by a liquid transfer system;

a third capillary or groove supplying dCTP into the reaction vessel by pressurizing or by a liquid transfer system;

a forth capillary or groove supplying dTTP into the reaction vessel by pressurizing or by a liquid transfer system; and

a detector monitoring synthesis of a strand complementary to a template nucleic acid by detecting chemiluminescence which arises from reaction with ATP and luciferin in the presence of luciferase at the reaction vessel the ATP being converted from pyrophosphate produced from the synthesis which uses one of the dATP, the dGTP, the dCTP and the dTTP,

wherein the template nucleic acid is set in the reaction vessel.

2. The system according to claim 1, wherein the reaction vessel and the first, second, third and fourth capillaries or groves are incorporated into one module.

3. The system according to claim 1, wherein the first, second, third and fourth capillaries or grooves are introduced into a top of the reaction vessel.

4. The system according to claim 1, further comprising dNTP reservoirs each containing one of the dATP, the dGTP, the dCTP and the dTTP and being pressure-controlled to supply one of the dATP, the dGTP, the dCTP and the dTTP contained therein intermittently and repeatedly into the reaction vessel, and an apparatus for controlling electric field between each of the dNTP reservoirs and the reaction vessel.

5. The system according to claim 1, wherein each of the first, second, third and fourth capillaries or grooves has an inner diameter of less than 0.2 mm or a cross-section area less than 0.04 mm², at an inlet of the reaction vessel.

6. The system according to claim 1, wherein each of the first, second, third and fourth capillaries or grooves has an inner diameter of less than 0.1 mm or a cross-section area less than 0.01 mm², at an inlet of the reaction vessel.

7. The system according to claim 5, further comprising reagent reservoirs, and reaction solutions each containing one of the dATP, the dGTP, the dCTP and the dTTP being introduced from the reagent reservoirs into the reaction vessel via the first, second, third and fourth capillaries or grooves connected at bottom of the reaction vessel.

8. The system according to claim 5, further comprising a supply unit set on top of the reaction vessel for supplying reaction solutions containing one of the dATP, the dGTP, the dCTP and the dTTP to the reaction vessel and a reaction vessel unit including the reaction vessel, the supply unit and the reaction vessel unit are separable, and the reaction solutions are alternatively and repeatedly supplied from the supply unit via the first, second, third and fourth capillaries or grooves.

9. A nucleic acid analyzing system comprising:

a reaction vessel;

a first capillary or groove supplying dATP into the reaction vessel by pressurizing or by a liquid transfer system;

a second capillary or grove supplying dGTP into the reaction vessel by pressurizing or by a liquid transfer system;

a third capillary or groove supplying dCTP into the reaction vessel by pressurizing or by a liquid transfer system;

a forth capillary or groove supplying dTTP into the reaction vessel by pressurizing or by a liquid transfer system; and

a detector monitoring synthesis of a strand complementary to a template nucleic acid by detecting chemiluminescence which arises from reaction with ATP and luciferin in the presence of luciferase at the reaction vessel the ATP being converted from pyrophosphate produced from the synthesis which uses one of the dATP, the dGTP, the dCTP and the dTTP,

wherein the template nucleic acid is set in the reaction vessel.

10. The DNA analyzing, system according to claim 9, wherein the detector is capable of distinguishing at least two positions emitting the chemiluminescence.

11. The DNA analyzing system according to claim 9, wherein the detector is an area sensor.

12. The DNA analyzing system according to claim 9, wherein the reaction vessel is selectively shifted relative to the detecting device.

13. The DNA analyzing system according to claim 9, wherein the reaction solutions are supplied substantially simultaneously and independently to the reaction vessel by an ink-jet method.

14. The DNA analyzing system according to claim 12, wherein the detector is a photon multiplier tube or an avalanche photodiode.