Device and a method for thermal sensing

• US 6,843,596 B2
• Filed: 03/07/2003
• Issued: 01/18/2005
• Est. Priority Date: 12/19/1997
• Status: Expired due to Fees

• Current Assignee: TTP Labtech Limited
• Original Assignee: Vivactis NV
• Inventors: Verhaegen, Katarina
• Primary Examiner(s): Nelms, David
• Assistant Examiner(s): Nguyen, Dao H.
• Application Number:
  US10/385,410
• Publication Number:
  US 20030152128A1
• Time in Patent Office: 683 Days
• Field of Search: 257/414, 257/467, 257/469, 374/10, 374/12, 374/30, 374/109, 438/54, 438/739, 438/49, 438/743, 438/756
• US Class Current: 374/10
• CPC Class Codes: G01K 7/028 using microstructures, e.g. made of silicon
• CPC Class Codes: Y10S 257/93 Thermoelectric, e.g. peltier effect cooling

Abstract

A device for thermal sensing is based on only one thermopile. The junctions of the thermopile are coupled thermally to a first region which includes a first substance while the hot junctions of the thermopile are coupled thermally to a second region which includes a second substance. The first and second regions are separated and thermally isolated from each other. The device can further include a membrane to thermally and electrically isolate the thermopile and to mechanically support the thermopile.

Claims

1. A method for detecting an effect on a second substance comprising:

providing a device, the device comprising

a support;

a membrane having a first surface and a second surface, the membrane mechanically supported by the support on at least one point on one of the first surface or the second surface,

at least one pair of devices, wherein one device of the pair of devices is located in a first region of the first surface of the membrane and the other device of the pair of devices is located in a second region of the first surface of the membrane, wherein the membrane thermally and electrically isolates the first region and the second region, and wherein each device of the pair of devices yields an electrical output signal in response to having an input signal of the thermal type,

a first supply for bringing a first substance in thermal contact with the first region, and

a second supply for bringing a second substance in thermal contact with the second region, wherein the first substance is separated and thermally isolated from the second substance;

providing a first substance in the first region;

providing a second substance in the second region;

contacting the first substance in the first region and the second substance in the second region with a third substance; and

determining whether there is a relative change of the electrical output signals of the device.

2. A method as recited in claim 1 wherein said first substance is a reference substance and wherein said second substance is a substance under test.

3. A method as recited in claim 1 wherein said first substance is a group of reference cells and said second substance is a group of cells under test.

4. A method as recited in claim 3 wherein said second substance is a group of modified cells.

5. A method as recited in claim 3 wherein said second substance is a group of genetically engineered cells.

6. A method as recited in claim 1 wherein said relative change of the electrical output signal is correlated to a relative change in thermal input originating from the two different regions.

7. A method as recited in claim 1 further comprising determining a relation between the relative change in the electrical output signal and the third substance acting on said second substance.

8. A method as recited in claim 1 wherein said third substance is selected from the group consisting of a chemical compound, a physical event and a biological species.

9. A method as recited in claim 1 wherein said at least one pair of devices forms a thermocouple, wherein one device of said at least one pair of devices is the hot junction and the other device of said at least one pair of devices is the cold junction of said thermocouple.

10. A method for detecting an effect of a cell affecting agent on a cell using a thermopile having a hot junction and a cold junction, the method comprising:

providing reference cells in a cold junction of a thermopile, with said cold junction located on a membrane above an opening;

providing genetically modified cells in a hot junction of the thermopile, the genetically modified cells expressing a cell affecting agent target;

contacting the cells in the cold junction and the cells in the hot junction with a candidate agent; and

monitoring relative heat differences between the hot junction and the cold junction wherein the relative heat difference is indicative of the contact between the cells in the hot junction and the cells in the cold junction with the candidate agent.

11. A method for measuring physical and chemical processes accompanied by changes in heat, the method comprising:

thermally coupling a first substance to a cold junction of a thermopile;

thermally coupling a second substance to a hot junction of the thermopile with the hot junction located on a membrane above an opening in a supportive substrate; and

monitoring the differential voltage produced by the thermopile, wherein the differential voltage is indicative of a change in heat of one of the substances.

12. The method of claim 11 wherein said first substance comprises genetically modified cells

and said second substance comprises reference cells.

13. The method of claim 12, further comprising contacting the cells in the cold junction and the cells in the hot junction with a candidate agent.

14. A method as recited in claim 13 wherein said relative change of the electrical output signal of the electrical/thermal type is related to said parameter under test.

15. A method as recited in claim 13 wherein said parameter under test is a physical property of said second substance.

16. A method as recited in claim 13 wherein said parameter under test is the activity of micro-organisms in said second substance.

17. The method of claim 13 further comprising contacting the first substance and the second substance with a test substance.

18. A method for detecting a parameter under test comprising:

providing a device, the device comprising

a support,

a membrane having a first surface and a second surface, the membrane mechanically supported by the support on at least one point on one of the first surface or the second surface,

at least one pair of devices, wherein one device of the pair of devices is located in a first region of the first surface of the membrane and the other device of the pair of devices is located in a second region of the first surface of the membrane, wherein the membrane thermally and electrically isolates the first region and the second region, and wherein each device of the pair of devices yields an electrical output signal in response to having an input signal of the thermal type,

a first supply for bringing a first substance in thermal contact with the first region, and

a second supply for bringing a second substance in thermal contact with the second region, wherein the first substance is separated and thermally isolated from the second substance;

providing a first substance in the first region;

providing a second substance in the second region,

determining whether there is a relative change of the electrical output signal between the two regions.