Functional surface coating

• US 6,844,028 B2
• Filed: 06/25/2002
• Issued: 01/18/2005
• Est. Priority Date: 06/26/2001
• Status: Active Grant

• Current Assignee: Accelerate Diagnostics, Inc.
• Original Assignee: Accelr8 Technology Corporation
• Inventors: Lochhead, Michael J., Metzger, Steven W., Mao, Guoqiano
• Primary Examiner(s): CAMERON, ERMA C
• Application Number:
  US10/180,199
• Publication Number:
  US 20030022216A1
• Time in Patent Office: 938 Days
• Field of Search: 427/384
• US Class Current: 427/384
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]
• CPC Class Codes: Y10T 428/31612 As silicone, silane or siloxane
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 428/31678 Of metal

Abstract

Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group'"'"'s activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

Claims

1. A method of preparing a functional surface, said method comprising:

providing a substrate;

providing an effective amount of active component comprising a functional group, spacer group, and a binding group;

providing an effective amount of cross-linking component;

providing an effective amount of matrix-forming component;

affixing said active component, said cross-linking component and said matrix forming component onto said substrate, thereby forming a functional surface.

2. The method of claim 1, wherein said affixing further comprises coating said active component, said cross-linking component and said matrix forming component onto said substrate creating a coated substrate and curing said coated substrate.

3. The method of claim 2, wherein said curing is thermal activation.

4. The method of claim 1, wherein said functional group is biotin.

5. The method of claim 1, wherein said spacer group is polyethylene glycol.

6. The method of claim 1, wherein said binding group is a silanes.

7. The method of claim 1, wherein said binding group is an alkoxysilane.

8. The method of claim 1, wherein said cross-linking component is acrylates.

9. The method of claim 1, wherein said matrix-forming component is a polyoxyethylene-based surface-active substances.

10. The method of claim 1, wherein said matrix-forming component is polyoxyethylene sorbitan tetraoleate.