×

Ultrahigh resolution multicolor colocalization of single fluorescent probes

  • US 6,844,150 B2
  • Filed: 08/08/2001
  • Issued: 01/18/2005
  • Est. Priority Date: 08/24/2000
  • Status: Active Grant
First Claim
Patent Images

1. A method for the colocalization of two or more species of interest in a sample comprising:

  • a) exciting the species of interest with a single wavelength of light thereby causing the two or more species of interest to emit light of a distinctive emission characteristic, b) separating the distinctive emission characteristics of the two or more species of interest, c) detecting the emitted light from the two or more species of interest, d) moving the sample a predetermined distance, e) repeating steps a) through d) a predetermined number of times thereby creating a multitude of representations of the excitation point spread function (PSF), f) determine the geometric center of the representations of the excitation PSF for at least two species of the two or more species of interest, g) computing the distance between the geometric centers of the representations of the excitation PSF for the species of interest.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×