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Retentate chromatography and protein chip arrays with applications in biology and medicine

  • US 6,844,165 B2
  • Filed: 12/21/2000
  • Issued: 01/18/2005
  • Est. Priority Date: 06/20/1997
  • Status: Expired due to Term
First Claim
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1. A method for diagnosing a disease in a subject by detecting a plurality of diagnostic markers of the disease comprising the steps of:

  • a) contacting at least one adsorbent that is attached to a substrate with a biological sample from the subject to allow the at least one adsorbent to bind the diagnostic markers;

    b) washing the at least one adsorbent with at least one eluant to remove unbound material and to allow retention of the diagnostic markers by the at least one adsorbent;

    c) detecting retained diagnostic markers by desorption spectrometry; and

    d) providing a diagnosis based on detection of the plurality of diagnostic markers.

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