Retentate chromatography and protein chip arrays with applications in biology and medicine
First Claim
Patent Images
1. A method for diagnosing a disease in a subject by detecting a plurality of diagnostic markers of the disease comprising the steps of:
- a) contacting at least one adsorbent that is attached to a substrate with a biological sample from the subject to allow the at least one adsorbent to bind the diagnostic markers;
b) washing the at least one adsorbent with at least one eluant to remove unbound material and to allow retention of the diagnostic markers by the at least one adsorbent;
c) detecting retained diagnostic markers by desorption spectrometry; and
d) providing a diagnosis based on detection of the plurality of diagnostic markers.
1 Assignment
0 Petitions
Accused Products
Abstract
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
-
Citations
21 Claims
-
1. A method for diagnosing a disease in a subject by detecting a plurality of diagnostic markers of the disease comprising the steps of:
-
a) contacting at least one adsorbent that is attached to a substrate with a biological sample from the subject to allow the at least one adsorbent to bind the diagnostic markers;
b) washing the at least one adsorbent with at least one eluant to remove unbound material and to allow retention of the diagnostic markers by the at least one adsorbent;
c) detecting retained diagnostic markers by desorption spectrometry; and
d) providing a diagnosis based on detection of the plurality of diagnostic markers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
Specification