Microarrays and their manufacture

• US 6,846,635 B1
• Filed: 07/28/2000
• Issued: 01/25/2005
• Est. Priority Date: 07/30/1999
• Status: Expired due to Fees

• Current Assignee: Large Scale Proteomics Corporation
• Original Assignee: Large Scale Proteomics Corporation
• Inventors: Anderson, N. Leigh, Anderson, Norman G., Braatz, James A.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/628,339
• Time in Patent Office: 1,642 Days
• Field of Search: 385/12, 385/15, 385/123, 356/244, 422/58, 422/82.02, 422/82.11, 435/7.1, 435/287.1, 435/287.2, 435/288.7, 435/808, 436/164, 436/165, 436/172, 436/518, 436/527, 436/535, 436/805, 436/809
• US Class Current: 435/7.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00524 in the shape of fiber bundles
• CPC Class Codes: B01J 2219/00547 Bar codes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B01L 3/50857 : using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 2500/00 : Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/5436 : with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 435/808 : Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 442/20 : Coated or impregnated woven, knit, or nonwoven fabric which is not [a] associated with another preformed layer or fiber layer or, [b] with respect to woven and knit, characterized, respectively, by a particular or differential weave or knit, wherein the coating or impregnation is neither a foamed material nor a free metal or alloy layer

Abstract

Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.

Claims

1. An array comprising a plurality of cells each in a known location on the array, each cell containing an agent of interest immobilized in or on a length of fiber, wherein different cells contain different fibers or lengths of fiber which contains a different agent of interest immobilized therein or thereon, and wherein each agent of interest is located in a known cell.

2. The array of claim 1 wherein the array contains portions of each fiber prepared by cutting a section from said fiber.

3. The array of claim 2 wherein the cells contain one well or channel.

4. The array of claim 1 wherein the agent of interest is immobilized in or on a complete length of the fiber.

5. The array of claim 1 wherein an agent of interest is located in a predetermined cell.

6. A binding assay for detecting an analyte in a sample wherein said analyte binds to at least one agent of interest in an array comprising;

contacting a sample suspected of containing an analyte with the array of claim 1 under conditions permitting the binding of analyte to agent of interest,

detecting the presence or absence of binding between analyte and agent of interest in each cell in the array which binding produces a detectable result, and

determining the presence or absence of the analyte by the presence of any binding being detected at a predetermined cell of the array.

7. The binding assay of claim 6, further comprising;

adding a labeled detection agent capable of binding to cells having either analyte bound to agent of interest or cells not having the analyte so bound, but not both, and

detecting the presence of the labeled detection agent in one or more cells of the array.