Crude extract from Viscum album coloratum, and proteins and lectins isolated therefrom

• US 6,846,913 B1
• Filed: 07/27/2000
• Issued: 01/25/2005
• Est. Priority Date: 07/27/1999
• Status: Expired due to Term

• Current Assignee: Mistle Biotech Company Limited
• Original Assignee: Mistle Biotech Company Limited
• Inventors: Kim, Jongbae, Lee, Kwanhee, Doo, Myoungsool, Yoon, Taekjoon, Kwak, Jinhwan, Song, Seongkyu, Park, Choonho, Kang, Taebong, Suh, Byungsun, Song, Byeoungdoo
• Primary Examiner(s): Low, Christopher S. F.
• Assistant Examiner(s): KAM, CHIH MIN
• Application Number:
  US09/627,165
• Time in Patent Office: 1,643 Days
• Field of Search: 514/8, 514/2, 514/12, 530/396, 530/350, 530/387.1, 536/23.6, 536/23.1, 435/320.1, 435/252.1, 435/325
• US Class Current: 530/396
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/04 specific for metastasis
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/415 from plants

Abstract

Disclosed is an extract from Korean mistletoe KM-110, which is of immunity enhancement and activity against tumor metastasis and can be used as an adjuvant material for vaccines applicable for the induction of humoral and cell-mediated immunity. Also disclosed are its fractions, a protein fraction KM-AS, a lectin fraction KML-C, lectin components KML-IIU and KML-IIL, which both are further purified from lectin fraction KML-C, a protein KMHBP which is obtained through heparin binding chromatography eluting with NaCl from a fraction C-free AS which is a portion of the KM-AS free of KML-C, and a mixture KM of the KMHBP and the KML-C. They are revealed as to their roles in the humoral and cell-mediated immunity enhancement and antitumoral activity.

Claims

1. A method of effectuating antitumoral activity, comprising administering to an animal in need thereof an amount of a lectin isolated from Korean mistletoe effective to enhance an anti-tumoral immune response, to reduce the size of a tumor, to inhibit tumor growth, to inhibit metastasis of a tumor, or a combination thereof,

wherein the lectin is selected from the group consisting of KML-IIU having a molecular weight of 61.8 kD and KML-IIL having a molecular weight of 56.4 kD.

2. The method of claim 1, wherein said KML-IIU has the amino acid sequence SEQ ID NO:

16.

3. The method of claim 1, wherein said KML-IIL has the amino acid sequence SEQ ID NO:

14.