Microfluidic sequencing methods
First Claim
Patent Images
1. A method of sequencing a template nucleic acid, the method comprising:
- mixing the template nucleic acid and one or more sequencing reagents in a microfluidic channel or chamber, whereby the template nucleic acid and the one or more sequencing reagents interact in the channel or chamber to form one or more sequencing products;
wherein the sequencing reagent comprises a primer, wherein the primer is delivered to the chamber or channel from a set of primers comprising a subset of all possible primers of a given length; and
, detecting the one or more sequencing products, thereby determining at least a subsequence of the template nucleic acid.
1 Assignment
0 Petitions
Accused Products
Abstract
Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems of are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.
123 Citations
49 Claims
-
1. A method of sequencing a template nucleic acid, the method comprising:
-
mixing the template nucleic acid and one or more sequencing reagents in a microfluidic channel or chamber, whereby the template nucleic acid and the one or more sequencing reagents interact in the channel or chamber to form one or more sequencing products;
wherein the sequencing reagent comprises a primer, wherein the primer is delivered to the chamber or channel from a set of primers comprising a subset of all possible primers of a given length; and
,detecting the one or more sequencing products, thereby determining at least a subsequence of the template nucleic acid. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
-
-
2. A method of sequencing a template nucleic acid, the method comprising:
-
mixing the template nucleic acid and one or more sequencing reagents in a microfluidic channel or chamber, whereby the template nucleic acid and the one or more sequencing reagents interact in the channel or chamber to form one or more sequencing products; and
,detecting the one or more sequencing products, thereby determining at least a subsequence of the template nucleic acid;
wherein detecting the one or more sequencing products comprises separating the sequencing products by size by electrophoresis in a microfluidic channel to produce a plurality of size separated sequencing products, and detecting the size separated sequencing products. - View Dependent Claims (29)
-
Specification