Modified barley α-glucosidase

• US 6,849,439 B2
• Filed: 01/10/2002
• Issued: 02/01/2005
• Est. Priority Date: 01/10/2001
• Status: Active Grant

• Current Assignee: United States Department Of Agriculture, Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: United States Department Of Agriculture, Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Henson, Cynthia A., Muslin, Elizabeth H., Clark, Suzanne E.
• Primary Examiner(s): Rao, Manjunath N.
• Application Number:
  US10/043,418
• Publication Number:
  US 20020184662A1
• Time in Patent Office: 1,118 Days
• Field of Search: 435/4, 435/6, 435/69.1, 435/183, 435200 T-210, 435/440, 435/455, 435/468, 435/252.3, 435/320.1, 536/23.2 T
• US Class Current: 435/200
• CPC Class Codes: C12N 15/8242 with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 9/2408 acting on alpha -1,4-glucosidic bonds
• CPC Class Codes: C12N 9/2411 Amylases
• CPC Class Codes: C12N 9/2451 acting on alpha-1,6-glucosidic bonds

Abstract

Barley α-glucosidase is an important enzyme in the conversion of barley starch to fermentable sugars during the industrial production of ethanol, as in brewing and fuel ethanol production. The enzyme is, however, relatively thermolabile, a disadvantage for an enzyme useful in industrial processes which are preferably conducted at elevated temperatures. Site directed mutagenesis has been conducted to make mutant forms of barley α-glucosidase which have improved thermostability. The sites for this site-directed mutagenesis were selected by sequence comparisons with the sequences of other α-glucosidase proteins which are more thermostable. The recombinant mutant enzymes thus produced have been demonstrated to improve the thermostability of the enzyme.

Claims

1. A modified α

-glucosidase enzyme, the modified form differing from the wild-type barley α

-glucosidase by proline being substituted for the threonine residue found in the wild-type protein in the amino acid sequence Val-Asn-Phe-Thr, which are amino acids 337 through 340 of SEQ ID NO;

1, the threonine located at residue 340 in SEQ ID NO;

1, the modified enzyme retaining activity at a higher temperature than the wild-type enzyme.

2. A modified α

-glucosidase enzyme, the modified enzyme differing from the wild-type barley α

-glucosidase by an amino acid modification which confers thermal stability on the modified enzyme so that the modified enzyme retains enzymatic activity at a higher temperature than the wild-type enzyme, the modification being selected from the group consisting of adding a proline and deleting an aspartate at residue 101, deleting an aspartate at residue 105, deleting an aspartate at residue 369, adding N-glycosylation site and deleting an aspartate at residue 372, adding N-glycosylation site to residue 463, deleting an aspartate at residue 508, and adding N-glycosylation site and deleting an aspartate at residue 694, the residues referring to SEQ ID NO;

1, and the residue positions determined by best-fit alignment of amino acid sequences to SEQ ID NO;

1.