Implantation of biological pacemaker that is molecularly determined

• US 6,849,611 B2
• Filed: 06/06/2001
• Issued: 02/01/2005
• Est. Priority Date: 06/06/2001
• Status: Expired due to Term

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University), The Research Foundation for The State University of New York (State University of New York)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University), The Research Foundation for The State University of New York (State University of New York)
• Inventors: Cohen, Ira S., Rosen, Michael R., Robinson, Richard B., Yu, Han-Gang
• Primary Examiner(s): Priebe, Scott D.
• Assistant Examiner(s): Whiteman, Brian
• Application Number:
  US09/875,388
• Publication Number:
  US 20020187948A1
• Time in Patent Office: 1,336 Days
• Field of Search: 435/455, 435/456, 435/325, 424/93.2, 424/93.21, 514/44
• US Class Current: 514/44.00R
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/177 Receptors; Cell surface antigens; Cell surface determinants
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61K 48/005 characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/06 Antiarrhythmics
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2710/10343 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2800/30 Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Abstract

This invention provides for a method of inducing a current in the heart in a subject which comprises contacting a cell of the heart of a subject with a compound in a sufficient amount to induce a current in the cell of the heart of a subject. This invention also provides a method of treating a cardiac condition in a subject which comprises contacting a cell of the heart of the subject with a compound in an amount sufficient to increase the current expression of the cell, thereby treating the cardiac condition in the subject.

Claims

1. A method of increasing the heart rate of a subject having a cardiac condition which comprises contacting a cell of the heart of the subject by injection or catheterization with a composition comprising a nucleic acid encoding MiRP1 and HCN2 in an amount sufficient to increase the pacemaker current expression of the cell, thereby increasing the heart rate.

2. The method of claim 1, wherein the subject is a mammal.

3. The method of claim 2, wherein the mammal is a human.

4. The method of claim 1, wherein the cell is a cardiac myocyte.

5. The method of claim 1, wherein the method is performed on a subject having a cardiac condition selected from the group consisting of bradycardia, complete atrioventricular block, incomplete atrioventricular block, heart block and sinus node dysfunction.

7. The method of claim 1, wherein the composition is an adenovirus, a virus or a liposome.

6. A method of inducing a pacemaker current in a cell of the heart of a subject which comprises contacting a cell of the heart of the subject by injection or catheterization with a compound comprising a nucleic acid encoding HCN2 and MiRP1 in a sufficient amount to induce the pacemaker current in the cell of the heart of the subject, thereby inducing a pacemaker current in the cell of the heart of the subject.