Ocular analyte sensor

• US 6,850,786 B2
• Filed: 09/03/2003
• Issued: 02/01/2005
• Est. Priority Date: 08/26/1999
• Status: Expired due to Term

• Current Assignee: EyeSense AG
• Original Assignee: Novartis Ag
• Inventors: March, Wayne Front
• Primary Examiner(s): Winakur, Eric F.
• Assistant Examiner(s): Kremer, Matthew
• Application Number:
  US10/655,502
• Publication Number:
  US 20040059207A1
• Time in Patent Office: 517 Days
• Field of Search: 600/319, 600/318, 600/321, 600/322, 600/329, 600/317
• US Class Current: 600/321
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/411 Detecting or monitoring allergy or intolerance reactions to an allergenic agent or substance
• CPC Class Codes: Y10S 977/905 Specially adapted for travel through blood circulatory system

Abstract

An ophthalmic lens comprising a receptor moiety can be used to determine the amount of an analyte in an ocular fluid. The receptor moiety can bind either a specific analyte or a detectably labeled competitor moiety. The amount of detectably labeled competitor moiety which is displaced from the receptor moiety by the analyte is measured and provides a means of determining analyte concentration in an ocular fluid, such as tears, aqueous humor, or interstitial fluid. The concentration of the analyte in the ocular fluid, in turn, inidicates the concentration of the analyte in a fluid or tissue sample of the body, such as blood or intracellular fluid.

Claims

1. An ophthalmic sensor for detecting an analyte in an cular fluid, comprising:

an ophthalmic lens; and

one or more detection reagents for said analyte in and/or on said ophthalmic lens, wherein the detection reagents comprise a receptor moiety and a fluorescent moiety wherein the receptor moiety comprises an analyte/competitor moiety binding site at which the analyte can reversibly bind, and wherein the fluorescent moiety in cooperation with the receptor moiety provides a detection of the analyte.

2. The ophthalmic sensor of claim 1, wherein the opthalmic lens is selected from the group consisting of a contact lens, an intraocular lens, a subconjunctival lens, an intracorneal lens, and a shunt or implant that can rest in the cul de sac of an eye.

5. The ophthalmic sensor of claim 2, wherein the detection reagents are covalently bound to the lens material of the ophthalmic lens.

6. The ophthalmic sensor of claim 5, wherein the detection reagents are boronic acid derivatives.

7. The ophthalmic sensor of claim 6, wherein the boronic acid derivatives comprise a fluorescent moiety, a boronic acid and tertiary amine.

8. The ophthalmic sensor of claim 2, wherein the detection reagents are boronic acid derivatives.

9. The ophthalmic sensor of claim 8, wherein the boronic acid derivatives comprise a fluorescent moiety, a boronic acid and tertiary amine.

3. The ophthalmic sensor of clalm 2, wherein the analyte is selected from the group consisting of an electrolyte, a metallic element, a polypeptide hormone, a chronically administered medication, an acutely administered medication, small molecule hormone, a marker of inflammation, a marker of allergy, a lipid, a protein, marker of infection, and a metabolite.

4. The ophthalmic sensor of claim 3, wherein the ocular fluid is selected from the group consisting of tears, aqueous humor, and interstitiial fluid.

10. An analyte sensor system, comprising

(a) an ophthalmic lens for detecting an analyte in an ocular fluid, having detection reagent for said analyte, wherein the detection reagent comprises a receptor moiety and a fluorescent moiety, wherein the receptor moiety comprises an analyte/competitor moiety binding site at which the analyte can reversibly bind, and wherein the fluorescent moiety in cooperation with the receptor moiety provides a detectable signal indicative of the analyte;

(b) a detector configured to detect the detectable signal.

11. The analyte sensor system of claim 10, further comprising a transmitter coupled to the detector and configured to transmit to a pump a signal indicating whether the detectable signal is detected by the detector, wherein the pump is configured to vary a concentration of the analyte in a body fluid or tissue.

12. The analyte sensor system of claim 11, wherein the transmitter is contained in a personal accessory.

13. The analyte sensor system of claim 11, further comprising the pump.

14. The analyte sensor system of claim 11, wherein the transmitter is further configured to transmit the signal to the pump wirelessly.

15. The analyte sensor system of claim 10, wherein the ophthalmic lens is selected from the group consisting of a contact lens, an intraocular lens, a subconjunctival lens, an intracorneal lens and a shunt or implant that can rest in the cul e sac of an eye.

16. The analyte sensor system of claim 15, wherein the analyte is glucose and the body fluid is blood.

17. The ophthalmic sensor of claim 15, wherein the detection reagent is covalently bound to the lens material of the ophthalmic lens.

18. The ophthalmic sensor of claim 17, wherein the detection reagent is a boronic acid derivative.

19. The ophthalmic sensor of claim 18, wherein the boronic acid derivative comprises a fluorescent moiety, a boronic acid and tertiary amine.

20. A kit for detecting glucose in an ocular fluid, comprising:

a contact lens comprising a boronic acid derivative which can bind reversibly to glucose to provide a fluorescent signal; and

instructions for using the contact lens to detect glucose in the ocular fluid.

21. The kit of claim 20, wherein the boronic acid derivative comprises a fluorescent moiety, a boronic acid and tertiary amine.

22. The kit of claim 20, comprising a detector for detecting the fluorescent signal.