Method and apparatus for automatic analysis

• US 6,852,212 B2
• Filed: 10/02/2001
• Issued: 02/08/2005
• Est. Priority Date: 08/13/1997
• Status: Expired due to Fees

• Current Assignee: Lifescan Incorporated (Duv Holding Corporation)
• Original Assignee: Lifescan Incorporated (Duv Holding Corporation)
• Inventors: Maxwell, Ian Andrew, Hodges, Alastair McIndoe, Beck, Thomas William
• Primary Examiner(s): Nguyen, Nam
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/970,461
• Publication Number:
  US 20020117404A1
• Time in Patent Office: 1,225 Days
• Field of Search: 205/777.5, 205/789, 205/775, 204/403.01, 204/403.14, 204/403.12, 204/416, 204/400
• US Class Current: 205/775
• CPC Class Codes: G01N 27/4168 Oxidation-reduction potential, e.g. for chlorination of water water analysis G01N33/18
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

This invention relates to a method for analyzing the concentration of an analyte in a sample and to automatic analyzing apparatus. The invention will be described herein with particular reference to a method and apparatus for measuring the concentration of glucose or other analytes in blood but is not limited to that use.

Claims

1. A method for estimating the concentration of a reduced or oxidized form of a redox species in a liquid comprising the steps of:

(1) contacting an area of a first electrode with a sample of predetermined volume of the liquid,

(2) bringing a second electrode into a closely spaced relationship with the first electrode, thereby contacting the sample with an area of the second electrode spaced apart from the first, wherein step (2) is conducted after step (1),

(3) applying a potential between the electrodes while the electrodes are sufficiently closely spaced that reaction products formed at each electrode diffuse to the other electrode while the potential is applied,

(4) measuring or estimating a value indicative of the change in current as a function of time and a value indicative of the steady state current, and

(5) determining from said value, said current as a function of time, and said steady state current the concentration of reduced or oxidized form of the species in the liquid sample wherein at least one of the electrodes is in the form of a continuous strip.

2. A method according to claim 1 wherein at least one of the electrodes is preprinted with at least one reagent.

3. A method according to claim 1 wherein at least one electrode is covered with a layer which serves to define wells on the electrode surface.

4. Apparatus according to claim 1, further comprising a pipette for depositing a drop of a predetermined volume of sample on the first electrode.

5. The method according to claim 1, wherein the first electrode comprises a first metallized electrode layer, wherein the second electrode comprises a second metallized electrode layer, and wherein the first metallized electrode layer travels transversely of the second electrode layer.

6. The method according to claim 1, wherein the first electrode comprises a first metallized electrode layer, wherein the second electrode comprises a second metallized electrode layer, and wherein the first metallized electrode layer travels in a same direction as the second electrode layer.

7. An automatic analyzing apparatus comprising:

a first electrode,

means for placing a predetermined volume of a liquid sample in contact with the first electrode,

means for bringing a second electrode into a closely spaced relationship with the first electrode after a predetermined volume of a liquid sample has been brought in contact with the first electrode, thereby contacting the sample with the second electrode spaced from the first,

means for applying a potential between the electrodes, and

means for measuring the current as a function of time while the electrodes are in sufficiently close proximity that reaction products formed at one electrode diffuse to the other and achieve a steady state distribution wherein a volume intermediate the electrode surfaces when the current is measured is not contained by a cell.

8. Apparatus according to claim 7 wherein the first electrode further comprises a porous medium wherein the predetermined volume is immobilized.

9. Apparatus according to claim 7 wherein at least one of the electrodes is a palladium layer.

10. Apparatus according to claim 7 further comprising a pipette for depositing a predetermined volume of sample in contact on the first electrode.

11. Apparatus according to claim 7 further comprising means for depositing one or more reagents on one of the electrodes prior to placing the sample on the electrode.

12. Apparatus according to claim 7, wherein the first electrode further comprises a well defining layer, the well defining layer comprises an aperture, the aperture defining a well.

13. Apparatus according to claim 7, wherein the first electrode or the second electrode further comprises a predetermined quantity of a chemical reagent.

14. Apparatus according to claim 7, wherein at least one of the first electrode and the second electrode is in the form of a disposable probe.

15. Apparatus according to claim 7, wherein at least one of the first electrode and the second electrode is in the form of a retractable probe.

16. An automatic analyzing apparatus comprising:

a first electrode,

means for placing a predetermined volume of a liquid sample in contact with the first electrode,

means for bringing a second electrode into a closely spaced relationship with the first electrode after a predetermined volume of a liquid sample has been brought in contact with the first electrode, thereby contacting the sample with the second electrode spaced from the first,

means for applying a potential between the electrodes, and

means for measuring the current as a function of time while the electrodes are in sufficiently close proximity that reaction products formed at one electrode diffuse to the other and achieve a steady state distribution wherein at least one electrode is in the form of a continuous strip.

17. An automatic analyzing apparatus comprising:

a first electrode,

means for placing a predetermined volume of a liquid sample in contact with the first electrode,

means for bringing a second electrode into a closely spaced relationship with the first electrode after a predetermined volume of a liquid sample has been brought in contact with the first electrode, thereby contacting the sample with the second electrode spaced from the first,

means for applying a potential between the electrodes, and

means for measuring the current as a function of time while the electrodes are in sufficiently close proximity that reaction products formed at one electrode diffuse to the other and achieve a steady state distribution wherein at least one electrode is on a flexible backing.

18. An automatic analyzing apparatus comprising:

a first electrode,

means for placing a predetermined volume of a liquid sample in contact with the first electrode,

means for bringing a second electrode into a closely spaced relationship with the first electrode after a predetermined volume of a liquid sample has been brought in contact with the first electrode, thereby contacting the sample with the second electrode spaced from the first,

means for applying a potential between the electrodes, and

means for measuring the current as a function of time while the electrodes are in sufficiently close proximity that reaction products formed at one electrode diffuse to the other and achieve a steady state distribution wherein at least one of the first electrode and the second electrode is mounted on a carousel.