Multi-well apparatus

• US 6,852,290 B2
• Filed: 03/08/2002
• Issued: 02/08/2005
• Est. Priority Date: 03/08/2001
• Status: Expired due to Fees

• Current Assignee: Exelixis Incorporated
• Original Assignee: Exelixis Incorporated
• Inventors: Swartwood, Troy M., Hager, David C., Morrissey, Michael, Kearney, Patrick, Leahy, James W., Mercer, Robert D., Donaldson, Jeffrey D., Keller, Douglas O.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): GORDON, BRIAN R
• Application Number:
  US10/094,253
• Publication Number:
  US 20020125197A1
• Time in Patent Office: 1,068 Days
• Field of Search: B01/D00.9/05, 738/634.1, 738/637.4, 738/644.1, 422/130, 422/131, 422 99-104, 436/177, 435/287.6, 435/258.4, 435/309.1, 435/309.2, 435/284.1, 210/247, 210/233, 210/323.2, 604/520, 604/86, 604/139, 604/148, 604158-162, 604/87, 600575-579
• US Class Current: 506/33
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00283 Reactor vessels with top opening
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00313 the reactor vessels being formed by arrays of wells in blocks
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00319 the blocks being mounted in stacked arrangements
• CPC Class Codes: B01J 2219/00333 Closures attached to the reactor vessels
• CPC Class Codes: B01J 2219/00416 Vacuum
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
• CPC Class Codes: B01L 3/5635 connecting two containers face to face, e.g. comprising a filter
• CPC Class Codes: C40B 50/08 Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A multi-well assembly according to one embodiment comprises a multi-well block and a guide plate. The multi-well block defines a plurality of wells, with each well having a fluid-impermeable bottom surface. The guide plate defines a plurality of fluid passageways corresponding to the wells of the multi-well block. The guide plate is configured such that, whenever the guide plate is registered with the multi-well block, fluid communication is established between each well and an associated fluid passageway.

Claims

1. A multi-well assembly, comprising:

a multi-well block having a plurality of wells, each well having an open top and a fluid-impermeable bottom surface; and

a guide plate defining a plurality of fluid passageways, each fluid passageway corresponding to a respective well of the multi-well block, the guide plate being configured such that registering the guide plate with the bottom of the multi-well block establishes fluid communication between each well and an associated fluid passageway to allow fluid to flow from each well into an associated fluid passageway, wherein the guide plate has upwardly extending integral projections that are non-removable from the guide plate, wherein the projections extend through and open the bottom surfaces of the wells whenever the guide plate is registered with the multi-well block;

wherein the bottom surfaces of the wells are impermeable to a fluid with which the multi-well block is used whenever the guide plate is not registered with the multi-well block.

2. The assembly of claim 1, wherein each projection is configured to perforate the bottom surface of a well.

3. The assembly of claim 1, further comprising a filter disposed in each well.

4. The assembly of claim 1, further comprising a receptacle block having a plurality of wells each corresponding to a respective fluid passageway of the guide plate such that, whenever the receptacle block is registered with the guide plate and the guide plate is registered with the multi-well block, fluid communication is established between each well of the multi-well block, a respective fluid passageway of the guide plate, and a respective well of the receptacle block.

5. The assembly of claim 1, further comprising a cover for covering the open tops of the wells of the multi-well block.

6. The assembly of claim 1, wherein each projection is formed generally in the shape of an ungula having two non-parallel, upwardly facing angled surfaces intersecting at a cutting edge for perforating the bottom surface of a respective well.

7. The assembly of claim 4, wherein the receptacle block has a substantially similar construction to that of the multi-well block.

8. A multi-well assembly, comprising:

a multi-well block having a plurality of wells, each well having an open top and a fluid-impermeable bottom surface; and

a guide plate defining a plurality of fluid passageways, each fluid passageway corresponding to a respective well of the multi-well block, the guide plate being configured such that, whenever the guide plate is registered with the bottom of the multi-well block, fluid communication is established between each well and an associated fluid passageway to allow fluid to flow from each well into an associated fluid passageway, wherein the guide plate has upwardly extending projections that extend through and open the bottom surfaces of the wells whenever the guide plate is registered with the multi-well block;

wherein the guide plate comprises;

a body having generally flat first and second opposed major surfaces, the projections depending from the first major surface; and

a plurality of outlet spouts depending from the second major surface;

wherein each of said fluid passageways extends through a respective projection and a respective outlet spout.

9. The assembly of claim 8, wherein the guide plate further comprises a plurality of first walls depending from the first major surface and a plurality of second walls depending from the second major surface, each first wall being concentrically disposed about a respective projection and each second wall being concentrically disposed about a respective outlet spout.

10. The assembly of claim 9, wherein each first wall is configured to matingly fit and form a fluid-tight seal around a bottom portion of a corresponding well of the multi-well block, thereby minimizing cross-contamination between fluids flowing from different wells.

11. The assembly of claim 8, further comprising a receptacle block having a plurality of wells each corresponding to a respective outlet spout of the guide plate such that, whenever the receptacle block is registered with the guide plate and the guide plate is registered with the multi-well block, each outlet spout extends into a respective well of the receptacle block so as to establish fluid communication between each well of the multi-well block, a respective fluid passageway of the guide plate, and a respective well of the receptacle block.

12. A multi-well assembly comprising:

a first plate having a plurality of wells, each well having a bottom end and an open top end opposite the bottom end for introducing fluid into the wells; and

a second plate having first and second opposed major surfaces, the first surface having a plurality of upwardly extending fluid conduits, the second surface having a plurality of downwardly extending outlet spouts, each outlet spout being in fluid communication with a respective fluid conduit;

wherein, whenever the first plate is registered with the second plate, each fluid conduit extends upwardly through the bottom end of a respective well to receive fluid therefrom.

13. The assembly of claim 12, wherein:

the second plate comprises a unitary structure and the first and second surfaces of the second plate are generally flat;

each well has a fluid-tight bottom surface; and

whenever the first plate is registered with the second plate, each fluid conduit perforates a bottom surface of a respective well so that the fluid conduit is in fluid communication with the well.

14. The assembly of claim 12, further comprising a receptacle block having a plurality of containers, each outlet spout being configured to extend into a respective container of the receptacle block.

15. The assembly claim 12, wherein, whenever the first plate is registered with the second plate, each well and a corresponding fluid conduit define a substantially fluid-tight passageway.

16. The assembly of claim 12, wherein each fluid conduit comprises a respective projection formed with a longitudinally extending channel.

17. The assembly of claim 12, wherein the second plate comprises an upwardly extending wall circumscribing each fluid conduit, each wall having an inner surface and each fluid conduit having an outer surface, the inner surface of each wall being configured to matingly fit around a lower portion of a respective well whenever the first plate is registered with the second plate, the entire inner surface of each wall being spaced radially outwardly from the outer surface of a respective fluid conduit so as to define a space therebetween for receiving the lower portion of a respective well.

18. A multi-well testing apparatus, comprising:

a multi-well device comprising a plurality of wells each having a liquid-impervious lower surface and an opening spaced from the lower surface for introducing liquid into the wells; and

a guide tray comprising a plurality of fluid passageways, each fluid passageway corresponding to a well of the multi-well device, the guide tray comprising means for fluidly connecting each fluid passageway with a corresponding well upon the guide tray is being positioned underneath and registered with the multi-well device, the guide tray comprising a one-piece construction;

wherein the lower surfaces of the wells are impervious to a fluid with which the multi-well device is used whenever the guide tray is not registered with the multi-well device;

wherein said means for fluidly connecting each fluid passageway with a corresponding well comprises a plurality of upwardly extending projections configured to perforate the lower surfaces of the respective wells whenever the guide tray is registered with the multi-well device.

19. The apparatus of claim 18, further comprising a respective filter disposed in each well.

20. The apparatus of claim 18, wherein the fluid passageways extend through respective projections and the guide tray further comprises an upwardly extending wall circumscribing each projection, each wall being configured to press-fit and form a fluid-tight seal around a lower portion of a respective well whenever the guide tray is registered with the multi-well device, the walls preventing cross-contamination between fluids flowing from different wells.

21. The apparatus of claim 18, further comprising a receptacle block comprising a plurality of wells each corresponding to a respective fluid passageway of the guide tray such that, whenever the receptacle block is registered with the guide tray and the guide tray is registered with the multi-well device, fluid communication is established between each well of the multi-well device, a respective fluid passageway of the guide tray, and a respective well of the receptacle block.

22. A guide plate for use with a multi-well device, the guide plate comprising:

a body having upper and lower major surfaces;

a plurality of projections depending from the upper major surface;

a plurality of outlet spouts depending from the lower major surface, each outlet spout being positioned below a respective projection;

a respective fluid passageway extending through each projection and outlet spout; and

a respective upwardly extending wall concentrically disposed about each projection;

wherein the guide plate comprises a unitary structure.

23. The guide plate of claim 22, wherein:

the upper major surface is generally flat and continuous between the walls; and

the lower major surface is generally flat and continuous between the outlet spouts.

24. The guide plate of claim 22, further comprising a respective downwardly extending wall concentrically disposed about each outlet spout wherein each outlet spout extends from the lower major surface a greater distance than its respective downwardly extending wall.

25. The guide plate of claim 22, wherein each projection defines a respective cutting surface.

26. A guide plate for use with a multi-well device having a plurality of wells, the guide plate comprising:

a body having first and second major surfaces that are generally flat and parallel to each other;

a plurality of projections depending from the first major surface, each projection being configured to perforate the bottom surface of a respective well of the multi-well device; and

a plurality of walls, each surrounding a respective projection and being configured to matingly fit around a bottom portion of a corresponding well of the multi-well device;

wherein each projection defines a passageway to receive the contents of a corresponding well of the multi-well device.

27. The guide plate of claim 26, further comprising a plurality of outlet spouts depending from the second major surface, wherein each passageway extends through a respective projection and a respective outlet spout.

28. The guide plate of claim 26, further comprising:

a plurality of outlet spouts extending from the second major surface opposite the projections; and

a plurality of walls extending from the second major surface and disposed about the outlet spouts, wherein each outlet spout extends from the second major surface a distance greater than its respective wall.

29. A guide plate for use with a multi-well device having a plurality of wells, the guide plate comprising:

a body having first and second major surfaces; and

a plurality of projections depending from one of the first and second major surfaces, each projection being configured to perforate the bottom surface of a respective well of the multi-well device;

wherein each projection is formed generally in the shape of an ungula comprising a generally cylindrical portion having two non-parallel, substantially flat upwardly facing angled surfaces intersecting at a cutting edge for perforating the bottom surface of a respective well.

30. A multi-well assembly comprising:

a multi-well block having a plurality of wells, each well having a fluid-impermeable bottom surface;

a guide plate defining a plurality of fluid passageways, each fluid passageway corresponding to a respective well of the multi-well block, the guide plate being configured such that, registering the guide plate with the bottom of the multi-well block;

establishes fluid communication between each well and an associated fluid passageway to allow fluid to flow from each well into an associated fluid passageway;

wherein the bottom surfaces of the wells are impermeable to a fluid with which the multi-well block is used when the guide plate is not registered with the multi-well block; and

a receptacle block having a plurality of wells each corresponding to a respective fluid passageway of the guide plate such that, whenever the receptacle block is registered with the guide plate and the guide plate is registered with the multi-well block, fluid communication is established between each well of the multi-well block, a respective fluid passageway of the guide plate, and a respective well of the receptacle block;

wherein the receptacle block has the same construction as the construction of the multi-well block.