Identifying a base in a nucleic acid
First Claim
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1. A method of detecting a mutation in a target nucleic acid sequence versus a known sequence comprising:
- a) exposing the target sequence to at least one known core sequence probe;
b) determining the binding affinity of the target sequence to the at least one known core sequence probe;
c) determining the binding affinity of the target sequence to at least one probe that has a single nucleotide variation as compared to the at least one known core sequence probe; and
d) comparing the binding affinity determined in step b) to the binding affinity determined in step c);
thereby detecting a mutation in a target nucleic acid sequence versus a known sequence.
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Abstract
Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.
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Citations
22 Claims
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1. A method of detecting a mutation in a target nucleic acid sequence versus a known sequence comprising:
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a) exposing the target sequence to at least one known core sequence probe;
b) determining the binding affinity of the target sequence to the at least one known core sequence probe;
c) determining the binding affinity of the target sequence to at least one probe that has a single nucleotide variation as compared to the at least one known core sequence probe; and
d) comparing the binding affinity determined in step b) to the binding affinity determined in step c);
thereby detecting a mutation in a target nucleic acid sequence versus a known sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22)
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21. A method of claim 25, wherein said at least one probe having a single nucleotide variation as compared to the at least one known core sequence probe is made by substituting A, C, T, U or G at each position of the at least one known core sequence probe.
Specification