Amplification and detection reagents for HIV-1

• US 6,852,491 B2
• Filed: 09/04/2001
• Issued: 02/08/2005
• Est. Priority Date: 09/04/2001
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Abravaya, Klara, Harris, Barbara J., Esping, Claudia A., Gorzowski, Jacek J., Swanson, Priscilla, Devare, Sushil G., Hackett, John R. Jr., Tang, Ning
• Primary Examiner(s): STUCKER, JEFFREY J
• Application Number:
  US09/945,943
• Publication Number:
  US 20030148280A1
• Time in Patent Office: 1,253 Days
• Field of Search: 435/5, 435/6, 435/974, 536/23.72, 536/24.32
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS

Abstract

Oligonucleotide primer sets, probes, and combinations of the primer sets and probes are provided herein. These reagents are useful for amplifying and detecting HIV-1 target sequences in a test sample.

Claims

1. A probe selected from the group consisting of:

SEQ ID NO;

41;

SEQ ID NO;

42;

SEQ ID NO;

43;

SEQ ID NO;

44;

SEQ ID NO;

45;

SEQ ID NO;

46;

SEQ ID NO;

47;

SEQ ID NO;

48;

SEQ ID NO;

49;

SEQ ID NO;

58;

SEQ ID NO;

59;

SEQ ID NO;

60;

SEQ ID NO;

61;

SEQ ID NO;

62;

SEQ ID NO;

63;

SEQ ID NO;

64; and

SEQ ID NO;

65.

2. The probe of claim 1, wherein the probe is selected from the group consisting of:

SEQ ID NO;

41;

SEQ ID NO;

42;

SEQ ID NO;

45;

SEQ ID NO;

48; and

SEQ ID NO;

49.

3. A method for detecting the presence of HIV-1 in a test sample comprising:

contacting a test sample with amplification reagents;

placing the reaction mixture under amplification conditions to form an amplification product;

forming a hybrid between the amplification product and a probe selected from the group consisting of SEQ ID NO;

41;

SEQ ID NO;

42;

SEQ ID NO;

43;

SEQ ID NO;

44;

SEQ ID NO;

45;

SEQ ID NO;

46;

SEQ ID NO;

47;

SEQ ID NO;

48;

SEQ ID NO;

49;

SEQ ID NO;

58;

SEQ ID NO;

59;

SEQ ID NO;

60;

SEQ ID NO;

61;

SEQ ID NO;

62;

SEQ ID NO;

63;

SEQ ID NO;

64; and

SEQ ID NO;

65; and

detecting the hybrid as an indication of the presence of HIV-1 in the test sample.

4. The method of claim 3 wherein the hybrid is formed while the reaction mixture is under amplification conditions.

5. The method of claim 3,

wherein the probe is selected from the group consisting of SEQ ID NO:

41, SEQ ID NO;

42, SEQ ID NO;

43, SEQ ID NO;

44, SEQ ID NO;

45, SEQ ID NO;

46, SEQ ID NO;

47 and SEQ ID NO;

48, and

wherein the amplification reagents comprise SEQ ID NO;

28.

6. The method of claim 5, wherein the amplification reagents include a primers selected from the group consisting of:

SEQ ID NO;

28 and SEQ ID NO;

29, SEQ ID NO;

28 and SEQ ID NO;

30, SEQ ID NO;

28 and SEQ ID NO;

31, SEQ ID NO;

28 and SEQ ID NO;

32, and SEQ ID NO;

28 and SEQ ID NO;

33.

7. The method of claim 3, wherein the probe comprises SEQ ID NO:

49 and wherein the amplification reagents comprise SEQ ID NO;

28.

8. The method of claim 7, wherein the amplification reagents include a primers selected from the group consisting of:

SEQ ID NO;

28 and SEQ ID NO;

29, SEQ ID NO;

28 and SEQ ID NO;

30, SEQ ID NO;

28 and SEQ ID NO;

32, and SEQ ID NO;

28 and SEQ ID NO;

33.