Transdermal assay with magnetic clamp

• US 6,852,526 B2
• Filed: 10/28/2002
• Issued: 02/08/2005
• Est. Priority Date: 07/14/2000
• Status: Expired due to Fees

• Current Assignee: TransForm Pharmaceuticals, Inc. (Johnson & Johnson)
• Original Assignee: TransForm Pharmaceuticals, Inc. (Johnson & Johnson)
• Inventors: Cima, Michael J., Chen, Hongming, Gyory, J. Richard
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US10/282,505
• Publication Number:
  US 20030068614A1
• Time in Patent Office: 834 Days
• Field of Search: 435/33, 435/287.1, 435/287.9, 435/288.4, 435/297.1, 435/297.2, 435/297.5, 422/102, 422/101, 73/38, 359/398
• US Class Current: 435/288.4
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: G01N 13/00 Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects scanning-probe techniques or apparatus G01Q
• CPC Class Codes: G01N 2500/00 Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/5044 involving specific cell types
• CPC Class Codes: G01N 33/5088 of vertebrates

Abstract

The transdermal assay apparatus includes first, second, and third members. The first member has one or more sample surfaces, each of which is configured to receive a sample thereon. The second member defines one or more reservoirs, each of which has an opening on a surface of the second member. Each sample surface is substantially the same size as each opening. The transdermal assay apparatus also includes a magnetic clamp configured to clamp a tissue specimen between the sample surface and the opening. The magnetic clamp preferably includes a magnet having a strength that is selected based on the clamping force required between the first member and the second member. The invention also provides a method for using a transdermal assay apparatus.

Claims

1. A transdermal assay apparatus, comprising:

a first lower member having a sample surface configured to receive an array of samples thereon and a tissue specimen that overlays said array of samples;

a second upper member having an array of reservoirs each having an opening on the surface of said second member, wherein said sample surface array and said reservoir array are substantially aligned with one another when in use; and

wherein said apparatus further comprises multiple samples on said first lower member, said samples comprising a component-in-common and at least one additional component, wherein each sample differs from at least one other sample with respect to at least one of;

(i) the identity of the additional components, (ii) the ratio of the component-in-common to the additional component, or (iii) the physical state of the component-in-common.

2. The transdermal assay apparatus of claim 1, wherein said sample surface and said opening are of substantially equal size.

3. The transdermal assay apparatus of claim 1, wherein in use said sample surface and said opening are substantially aligned with one another along a line substantially perpendicular with said sample surface.

4. The transdermal assay apparatus of claim 1, wherein said reservoir is configured to receive at least one fluid medium therein.

5. The transdermal assay apparatus of claim 1, further comprising a magnetic clamp configured to clamp said tissue specimen between said sample surface and said opening.

6. The transdermal assay apparatus of claim 5, wherein said magnetic clamp comprises:

at least one magnet coupled to said first member; and

at least one ferrous insert coupled to said second member.

7. The transdermal assay apparatus of claim 5, wherein said magnetic clamp comprises:

at least one magnet coupled to said first member; and

at least one magnetic insert coupled to said second member.

8. The transdermal assay apparatus of claim 5, wherein said magnetic clamp comprises:

at least one magnet coupled to said first member; and

at least a portion of said second member made from a ferrous material.

9. The transdermal assay apparatus of claim 5, wherein said magnetic clamp comprises:

at least a portion of said first member made from a magnetic material; and

at least a portion of said second member made from a ferrous material.

10. The transdermal assay apparatus of claim 5, wherein said magnetic clamp comprises:

at least a portion of said first member made from a magnetic material; and

at least a portion of said second member made from a magnetic material.

11. The transdermal assay apparatus of claim 5, further comprising a third member configured to clamp said first member between said third member and said second member.

12. The transdermal assay apparatus of claim 11, wherein said magnetic clamp comprises:

at least one magnet coupled to said third member; and

at least one ferrous insert coupled to said second member.

13. The transdermal assay apparatus of claim 12, wherein said third member comprises a cavity for receiving said at least one magnet therein.

14. The transdermal assay apparatus of claim 11, wherein said magnetic clamp comprises:

at least one magnet coupled to said third member; and

at least one magnetic insert coupled to said second member.

15. The transdermal assay apparatus of claim 11, wherein said magnetic clamp comprises:

at least one magnet coupled to said third member; and

at least a portion of said second member made from a ferrous material.

16. The transdermal assay apparatus of claim 11, wherein said magnetic clamp comprises:

at least a portion of said third member made from a magnetic material; and

at least a portion of said second member made from a ferrous material.

17. The transdermal assay apparatus of claim 11, wherein said magnetic clamp comprises:

at least a portion of said third member made from a magnetic material; and

at least a portion of said second member made from a magnetic material.

18. The transdermal assay apparatus of claim 11, wherein:

said first member includes at least one alignment hole; and

said third member comprises at least one alignment post configured to mate with said at least one alignment hole.

19. The transdermal assay apparatus of claim 11, wherein said third member is made from a material selected from a group consisting of:

aluminum, steel, brass, plastic, ceramic, and any combination of the aforementioned.

20. The transdermal assay apparatus of claim 5, wherein said magnetic clamp includes a permanent magnet.

21. The transdermal assay apparatus of claim 5, wherein said magnetic clamp includes an electromagnet.

22. The transdermal assay apparatus of claim 5, wherein said magnetic clamp includes a magnet having a strength that is selected based on the clamping force required between said first member and said second member.

23. The transdermal assay apparatus of claim 1, wherein said first and second members are made from a material selected from a group consisting of:

stainless steel, plastic, polycarbonate, glass, aluminum, brass, ceramic, and any combination of the aforementioned.

24. The transdermal assay apparatus of claim 1, wherein the component-in-common is a pharmaceutical, a dietary supplement, a nutraceutical, or an alternative medicine.

25. The transdermal assay apparatus of claim 24, (wherein the component-in-common is a pharmaceutical.

26. The transdermal assay apparatus of claim 1, wherein the additional component is an adhesive, an enhancer, an additive, a solvent, an excipient, or a combination thereof.

27. The transdermal assay apparatus of claim 26, wherein the additional component is an enhancer.

28. The transdermal assay apparatus of claim 27, wherein the enhancer is a chemical enhancer, a lipid permeation enhancer, a solubility enhancer, or a combination of enhancers.

29. The transdermal assay apparatus of claim 26, wherein the additional component is an adhesive.

30. The transdermal assay apparatus of claim 29, wherein the adhesive is a polyisobutylene, a silicone, or an acrylic adhesive.

31. The transdermal assay apparatus of claim 1, wherein said apparatus further comprises a solid source sample.

32. The transdermal assay apparatus of claim 1, wherein said apparatus further comprises a liquid source sample.

33. The transdermal assay apparatus of claim 1, wherein said apparatus further comprises a tissue specimen.

34. The transdermal assay apparatus of claim 33, wherein the tissue specimen comprises skin tissue.

35. The transdermal assay apparatus of claim 34, wherein the skin tissue comprises epidermis or stratum corneum.

36. The transdermal assay apparatus of claim 34, wherein the skin tissue comprises stratum corneum.

37. The transdermal assay apparatus of claim 34, wherein the skin tissue consists of stratum corneum.

38. The transdermal assay apparatus of claim 34, wherein the skin tissue is human skin tissue, animal skin tissue, or engineered skin tissue.

39. The transdermal assay apparatus of claim 33, wherein the tissue specimen is divided into a plurality of segments by mechanical cutting, scribing, laser cutting, scoring, or crimping.