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Endoglucanases

  • US 6,855,531 B2
  • Filed: 12/10/2001
  • Issued: 02/15/2005
  • Est. Priority Date: 03/17/1995
  • Status: Expired due to Fees
First Claim
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1. A detergent composition comprising a surfactant and a polypeptide exhibiting endoglucanase activity;

  • which is any of;

    (a) a polypeptide having an amino acid sequence comprising the sequence of SEQ ID NO;

    12;

    (b) a polypeptide encoded by a DNA sequence which hybridizes with SEQ ID NO;

    11 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 10081 under the following hybridization conditions;

    prehybridization and hybridization in a solution of 5×

    SSC, 5 ×

    Denhardt'"'"'s solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA for 12 hours at about 45°

    C., followed by washing in 2×

    SSC and 0.5% SDS at a temperature of 65°

    C.; and

    (c) a fragment of the sequence of SEQ ID NO;

    12 which has endoglucanase activity, wherein the detergent composition has a pH in the range of 7-11.

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