Endoglucanases

• US 6,855,531 B2
• Filed: 12/10/2001
• Issued: 02/15/2005
• Est. Priority Date: 03/17/1995
• Status: Expired due to Fees

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Lassen, Søren Flensted, Kauppinen, Markus Sakari
• Primary Examiner(s): Slobodyansky, Elizabeth
• Application Number:
  US10/007,521
• Publication Number:
  US 20030054539A1
• Time in Patent Office: 1,163 Days
• Field of Search: 435/209, 435/263, 435/264, 510/320, 510/321, 536/23.2
• US Class Current: 435/209
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C05F 11/08 Organic fertilisers containing added bacterial cultures, mycelia or the like
• CPC Class Codes: C05F 17/20 using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment use of invertebrates C05F17/05
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C11D 3/386 Preparations containing enzymes , e.g. protease or amylase
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: D06M 16/003 with enzymes or microorganisms
• CPC Class Codes: D06P 5/02 After-treatment
• CPC Class Codes: D06P 5/158 with other compounds
• CPC Class Codes: D21C 5/005 Treatment of cellulose-containing material with microorganisms or enzymes
• CPC Class Codes: D21H 17/005 Microorganisms or enzymes
• CPC Class Codes: D21H 21/10 Retention agents or drainage improvers
• CPC Class Codes: Y02P 20/145 the feedstock being materials of biological origin
• CPC Class Codes: Y02W 30/40 Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence

(SEQ ID NO:79)Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa1   2   3   4   5   6   7   8   9Xaa Cys Xaa Trp Xaa10  11  12  13  14
and a second amino acid sequence having the following sequence

Trp Cys Cys Xaa Cys (SEQ ID NO:80)1   2   3   4   5
wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

Claims

1. A detergent composition comprising a surfactant and a polypeptide exhibiting endoglucanase activity;

which is any of;

(a) a polypeptide having an amino acid sequence comprising the sequence of SEQ ID NO;

12;

(b) a polypeptide encoded by a DNA sequence which hybridizes with SEQ ID NO;

11 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 10081 under the following hybridization conditions;

prehybridization and hybridization in a solution of 5×

SSC, 5 ×

Denhardt'"'"'s solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA for 12 hours at about 45°

C., followed by washing in 2×

SSC and 0.5% SDS at a temperature of 65°

C.; and

(c) a fragment of the sequence of SEQ ID NO;

12 which has endoglucanase activity, wherein the detergent composition has a pH in the range of 7-11.

2. The detergent composition of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

12.

3. The detergent composition of claim 1, wherein the polypeptide consists of the amino acid sequence of SEQ ID NO:

12.

4. The detergent composition of claim 1, wherein the polypeptide is a fragment of SEQ ID NO:

12 and has endoglucanase activity.

5. The detergent composition of claim 1, wherein the polypeptide is a Thielavia polypeptide.

6. The detergent composition of claim 5, wherein the polypeptide is a Thielavia terrestris polypeptide.

8. The detergent composition of claim 1, wherein the polypeptide is encoded by a DNA sequence which hybridizes with the DNA sequence of SEQ ID NO:

11 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 10081 under the following hybridization conditions;

prehybuldizatlon and hybridization in a solution of 5×

SSC, 5×

Denhardt'"'"'s solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA for 12 hours at about 45°

C., followed by washing in 2×

SSC and 0.5% SDS at 70°

C.

9. The detergent composition of claim 1, wherein the polypeptide is encoded by DNA sequence which hybridizes with the DNA sequence of SEQ ID NO:

11 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 10081 under the following hybridization conditions;

prehybridization and hybridization in a solution of 5×

SSC, 5×

Denhardt'"'"'s solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA for 12 hours at about 45°

C., followed by washing in 2×

SSC and 0.5% SDS at 75°

C.

11. The detergent composition of claim 8, wherein the polypeptide is a Thielavia polypeptide.

12. The detergent composition of claim 9, wherein the polypeptide is a Thielavia polypeptide.

14. The detergent composition of claim 11, wherein the polypeptide is a Thielavia terrestris polypeptide.

15. The detergent composition of claim 12, wherein the polypeptide is a Thielavia terrestris polypeptide.

16. The detergent composition of claim 1, further comprising one or more additional enzymes.

17. The detergent composition of claim 1, further comprising a detergent builder or complexing agent.

18. The detergent composition of claim 1, further comprising a polymer.

19. The detergent composition of claim 1, further comprising a bleaching system.

20. A method of providing color clarification to laundry, comprising treating the laundry with a detergent composition of claim 1.

7. The detergent composition of 1, wherein the polypeptide is encoded by a DNA sequence which hybridizes with the DNA sequence of SEQ ID NO:

11 or the DNA sequence obtainable from the plasmid in Saccharomyces cerevisiae DSM 10081 under the following hybridization conditions;

prehybridization and hybridization in a solution of 5×

SSC, 5×

Denhardt'"'"'s solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA for 12 hours at about 45°

C., followed by washing in 2×

SSC and 0.5% SOS at 65°

C.

10. The detergent composition of claim 7, wherein the polypeptide is a Thielavia polypeptide.

13. The detergent composition of claim 10, wherein the polypeptide is a Thielavia terrestris polypeptide.