Device for performing an assay, a method for manufacturing said device, and use of a membrane in the manufacture of said device

• US 6,855,539 B2
• Filed: 11/27/2001
• Issued: 02/15/2005
• Est. Priority Date: 07/11/1997
• Status: Expired due to Fees

• Current Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Original Assignee: PamGene International BV (VitrOmics Healthcare Holding BV)
• Inventors: van Damme, Hendrik Sibolt, van Beuningen, Marinus Gerardus Johannus, Boender, Pieter Jacob, Kreuwel, Hermanus Johannes Maria, Kievits, Tim
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/997,213
• Publication Number:
  US 20020164584A1
• Time in Patent Office: 1,176 Days
• Field of Search: 422/57, 422/58, 435/5, 435/6, 435/7.1, 435/287.1, 435/287.2, 435/810, 436/518, 436/524
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: Y10S 436/81 : Tube, bottle, or dipstick

Abstract

Disclosed is a device useful for performing an assay comprising a substrate having interconnecting channels that open out onto a surface for sample application, the channels being provided in at least one cross-sectional area with a first binding substance capable of binding a particular analyte, the substrate being an electrochemically manufactured metal oxide membrane and containing the first binding substance within the through-going channels. Similar devices are also useful for chemical synthesis. Assay and chemical synthesis methods are also disclosed, as are kits for performing the assays or chemical syntheses.

Claims

1. A device for performing an assay, which device comprises a substrate having interconnected channels, said channels opening out on a surface for sample application, the channels in at least one area of the surface for sample application being provided with a first binding substance capable of binding to an analyte, wherein the substrate is an electrochemically manufactured metal oxide membrane and the first binding substance is within the interconnected channels in the substrate.

2. The device of claim 1, wherein the metal oxide membrane is comprised of aluminium oxide.

3. The device of claim 1, wherein the first binding substance is covalently bound to the substrate.

4. The device of claim 1, wherein the first binding substance is selected from the group consisting of an oligopeptide, a polypeptide, an oligonucleotide, a polynucleotide, a hapten, and a ligand for a receptor.

5. The device of claim 4, wherein the first binding substance is an oligopeptide.

6. The device of claim 5, wherein the oligopeptide comprises an immunogenic epitope.

7. The device of claim 5, wherein the oligopeptide comprises a portion of an HIV protein.

8. The device of claim 4, wherein the first binding substance is a polypeptide.

9. The device of claim 8, wherein the first binding substance is an antibody or an enzyme.

10. The device of claim 4, wherein the first binding substance is an oligonucleotide.

11. The device of claim 10, wherein the first binding substance comprises a portion of an HIV genome.

12. A kit comprising (a) the device of claim 1, and (b) a detection means for determining whether binding has occurred between the first binding substance and the analyte.

13. The kit of claim 12 wherein the detection means comprises a second binding substance provided with a label.

14. The kit of claim 13, wherein the label is capable of inducing a colored or infrared or ultraviolet reaction product, or capable of bio- or chemo- or photoluminescence.

15. The kit of claim 14, wherein the detection means uses an enzymatic reaction.

16. A device for performing a chemical synthesis, which device comprises a substrate having interconnected channels, said channels opening out on a surface, the channels in at least one area of the surface for reagent application being provided with a first reacting substance capable of reacting with a second reacting substance, wherein the substrate is an electrochemically manufactured metal oxide membrane and the first reacting substance is within the interconnected channels in the substrate.

17. The device of claim 16, wherein the first reacting substance is a polymer.

18. The device of claim 16, wherein the first reacting substance is covalently bound to the substrate.

19. The device of claim 17, wherein the polymer is capable of covalently binding to an organic molecule of less than 1000 Dalton.

20. The device of claim 17, wherein the polymer is capable of covalently binding to a modified amino acid useful for oligopeptide synthesis.

21. The device of claim 17, wherein the polymer is capable of covalently binding to a modified nucleotide useful for oligonucleotide synthesis.