Polymer matrix containing catalase co-immobilized with analytic enzyme that generates hydrogen peroxide
First Claim
1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of the catalase in a pregel solution which gels to form the polymer matrix ranges from about 100 units/mL to about 1000 units/mL.
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Abstract
Hydrogels containing catalase co-immobilized with an analyte-sensitive enzyme such as glucose oxidase are disclosed. The hydrogels may be pH-sensitive, and preferably are thin and lightly crosslinked. The catalase is present in concentrations ranging generally from 100 units/ml to about 1000 units/ml. These hydrogels have much faster swelling response times as compared to hydrogels without catalase, and are useful in biosensors and analyte-responsive drug delivery devices. The hydrogels also have an increased useful life, due to protection of the immobilize analyte-sensitive enzyme from degradation by hydrogen peroxide.
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Citations
12 Claims
- 1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of the catalase in a pregel solution which gels to form the polymer matrix ranges from about 100 units/mL to about 1000 units/mL.
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9. A method of making a polymer matrix containing catalase co-immoblized with an analytic enzyme which generates hydrogen peroxide for use in a biosensor or analyte-responsive drug delivery device, comprising the steps of:
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making a pregel solution containing an added analytic enzyme which generates hydrogen peroxide and catalase where the catalase is added at a concentration ranging from about 100 units of catalase/mL pregel solution to about 1000 units catalase/mL pregel solution; and
polymerizing the pregel solution to form a polymer matrix and co-immobilize the analytic enzyme with the catalase in the polymer matrix. - View Dependent Claims (10, 11, 12)
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Specification