Creatinine biosensor
First Claim
1. A method for producing biosensors comprising at least two enzymes for the amperometric determination of enzymatically degradable substances in biological liquids wherein the enzymes are immobilized on a working electrode, comprising applying an enzyme together with one or more surface-active substances in an aqueous solution on the working electrode, allowing said enzyme together with the one or more surface-active substances in the aqueous solution to dry, and chemically immobilizing the at least second enzyme thereupon.
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Abstract
The present invention generally relates to a method for producing biosensors and a biosensor for determination of creatinine. The biosensor comprises at least two enzymes, for the amperometric determination of enzymatically degradable substances in biological liquids, the enzymes being immobilized on a working electrode.
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Citations
13 Claims
- 1. A method for producing biosensors comprising at least two enzymes for the amperometric determination of enzymatically degradable substances in biological liquids wherein the enzymes are immobilized on a working electrode, comprising applying an enzyme together with one or more surface-active substances in an aqueous solution on the working electrode, allowing said enzyme together with the one or more surface-active substances in the aqueous solution to dry, and chemically immobilizing the at least second enzyme thereupon.
Specification