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Production of heterologous polypeptides in yeast

  • US 6,861,237 B2
  • Filed: 11/30/2001
  • Issued: 03/01/2005
  • Est. Priority Date: 11/30/2000
  • Status: Expired due to Fees
First Claim
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1. A process for making a heterologous, non-bacterial polypeptide or an intermediate therefore in yeast comprising (i) culturing a yeast strain comprising a polynucleotide sequence encoding the polypeptide or an intermediate therefore under suitable culture conditions for expression of the polypeptide or its intermediate, wherein the polynucleotide sequence encoding the polypeptide or its intermediate is expressed under transcriptional control of a yeast CIT1 promoter or a functional part or variant thereof;

  • and (ii) isolating the expressed product.

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