Translucent solid matrix assay device for microarray analysis

• US 6,861,251 B2
• Filed: 02/24/2003
• Issued: 03/01/2005
• Est. Priority Date: 02/24/2003
• Status: Expired due to Fees

• Current Assignee: Gentel Biosciences Inc. (Intuitive Biosciences, Inc.)
• Original Assignee: PriTest Inc.
• Inventors: Green, Lawrence R.
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): YANG, NELSON C
• Application Number:
  US10/373,546
• Publication Number:
  US 20040166494A1
• Time in Patent Office: 736 Days
• Field of Search: 422/50, 422/56, 422/57, 422 8206- 7, 422/100, 435/6, 435/69.1, 435/91.2, 435/174, 435/177, 435/287.1, 436/172, 436/180, 436/160
• US Class Current: 506/39
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 50/14 Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support

Abstract

The present invention concerns methods and compositions relating to matrix arrays. In certain embodiments, the arrays are translucent. In other embodiments, the arrays are reconfigurable. In preferred embodiments, the arrays are translucent and reconfigurable. Reconfigurable arrays may be produced using small linker molecules, such as aptamers or affibodies, attached to the array substrate. Preferably, the small linker molecules bind to an IgG specific portion of antibodies. Such arrays may be used to detect any target that binds selectively or specifically to an IgG, allowing great flexibility of use. Translucent matrix arrays may utilize a translucent, colloidal form of nitrocellulose to coat the array substrate.

Claims

1. A translucent matrix array comprising:

a) a substrate;

b) an optically clear layer of colloidal nitrocellulose coated on the substrate; and

c) one or more first probes attached to the colloidal nitrocellulose.

2. The translucent matrix array of claim 1, wherein the first probes are attached to the array in spots of about 300 μ

m in diameter.

3. The translucent matrix array of claim 2, wherein the probes attached to different spots on the array bind to different targets.

4. The translucent matrix array of claim 1, wherein the first probes are selected from the group consisting of antibodies, antibody fragments, FAb fragments, humanized antibodies, single-chain antibodies, chimeric antibodies, oligonucleotides, polynucleotides, nucleic acids, aptamers and affibodies.

5. The translucent matrix array of claim 1, wherein the substrate is a glass substrate.

6. The translucent matrix array of claim 1, wherein the substrate is a plastic substrate.

7. The translucent matrix array of claim 4, wherein the first probes are aptamers or affibodies.

8. The translucent matrix array of claim 7, wherein the first probes bind to antibodies, antibody fragments, FAb fragments, humanized antibodies, single-chain antibodies or chimeric antibodies.

9. The translucent matrix array of claim 8, wherein the first probes bind to IgG (immunoglobulin type G) antibodies.

10. The translucent matrix array of claim 4, wherein the first probes are antibodies.

11. The translucent matrix array of claim 10, wherein the first probes are anti-mouse antibodies.

12. The translucent matrix array of claim 1, wherein the first probes are attached directly to the nitrocellulose.

13. The translucent matrix array of claim 1, wherein the one or more first probes are capable of binding to one or more targets.

14. The translucent matrix array of claim 13, wherein the one or more targets are selected from the group consisting of a protein, peptide, carbohydrate, polysaccharide, glycoprotein, lipid, hormone, growth factor, cytokine, receptor, antigen, allergen, antibody, substrate, metabolite, cofactor, inhibitor, drug, pharmaceutical, nutrient, toxin, poison, explosive, pesticide, chemical warfare agent, biowarfare agent, biohazardous agent, infectious agent, prion, radioisotope, vitamin, heterocyclic aromatic compound, carcinogen, mutagen, narcotic, amphetamine, barbiturate, hallucinogen, waste product, contaminant, heavy metal, virus, bacterium, Salmonella, Streptococcus, Legionella, E. coli, Giardia, Cryptosporidium, Rickettsia, spore, mold, yeast, algae, amoebae, dinoflagellate, unicellular organism, pathogen and cell.

15. The translucent matrix array of claim 13, further comprising an optical detector aligned to detect fluorescent labels attached to the targets.

16. The translucent matrix array of claim 15, wherein the optical detector comprises a CMOS imager.

17. The translucent matrix array of claim 1, wherein the optically clear nitrocellulose layer transmits light signals without scatter or interference.