Electrochemical biosensor strip for analysis of liquid samples
First Claim
1. A biosensor strip for determining the concentration of an analyte in a sample of biological liquid, said biosensor strip comprising:
- (a) an electrode support having an electrode bearing major surface;
(b) a cover layer having an electrode-bearing major surface;
(c) a spacer layer interposed between said electrode support and said cover layer;
(d) a first electrode, said first electrode being a working electrode, said working electrode comprising working ink comprising (i) a reagent responsive to said analyte in said sample of said biological liquid and (ii) an electron mediator deposited on an electrically conductive material;
(e) a second electrode, said second electrode being a reference electrode, said reference electrode comprising (i) said reagent responsive to said analyte in said sample of said biological liquid and (ii) said electron mediator deposited on an electrically conductive material; and
(f) a third electrode, said third electrode being a counter electrode, said counter electrode comprising an electrically conductive material, said electrode-bearing major surface of said first electrode support facing said electrode-bearing major surface of said cover layer.
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Accused Products
Abstract
A biosensor in the form of a strip. In one embodiment, the biosensor strip comprises an electrode support, a first electrode, i.e., a working electrode, a second electrode, i.e., a counter electrode, and a third electrode, i.e., a reference electrode. Each of the electrodes is disposed on and supported by the electrode support. Each of the electrodes is spaced apart from the other two electrodes. The biosensor strip can include a covering layer, which defines an enclosed space over the electrodes. This enclosed space includes a zone where an analyte in the sample reacts with reagent(s) deposited at the working electrode. This zone is referred to as the reaction zone. The covering layer has an aperture for receiving a sample for introduction into the reaction zone. The biosensor strip can also include at least one layer of mesh interposed in the enclosed space between the covering layer and the electrodes in the reaction zone. This layer of mesh facilitates transporting of the sample to the electrodes in the reaction zone. In another embodiment, a biosensor strip can be constructed to provide a configuration that will allow the sample to be introduced to the reaction zone by action of capillary force. In this embodiment, the layer of mesh can be omitted. The invention also provides a method for determining the concentration of glucose in a sample of whole blood by using the biosensor of this invention.
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Citations
12 Claims
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1. A biosensor strip for determining the concentration of an analyte in a sample of biological liquid, said biosensor strip comprising:
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(a) an electrode support having an electrode bearing major surface;
(b) a cover layer having an electrode-bearing major surface;
(c) a spacer layer interposed between said electrode support and said cover layer;
(d) a first electrode, said first electrode being a working electrode, said working electrode comprising working ink comprising (i) a reagent responsive to said analyte in said sample of said biological liquid and (ii) an electron mediator deposited on an electrically conductive material;
(e) a second electrode, said second electrode being a reference electrode, said reference electrode comprising (i) said reagent responsive to said analyte in said sample of said biological liquid and (ii) said electron mediator deposited on an electrically conductive material; and
(f) a third electrode, said third electrode being a counter electrode, said counter electrode comprising an electrically conductive material, said electrode-bearing major surface of said first electrode support facing said electrode-bearing major surface of said cover layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 11, 12)
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10. The biosensor strip of claim wherein at least one of said three electrodes is disposed on said electrode support and at least one of said remaining two electrodes is disposed on said cover layer.
Specification