Automatic diagnostic apparatus

• US 6,866,821 B2
• Filed: 08/18/2003
• Issued: 03/15/2005
• Est. Priority Date: 03/29/1996
• Status: Expired due to Fees

• Current Assignee: BYK Gulden Italia S.p.A.
• Original Assignee: BYK Gulden Italia S.p.A.
• Inventors: Casalin, Paola, Lissandrello, Fabio, Coupe, Neville, Connolly, Patricia, Friedlander, Uri
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US10/642,498
• Publication Number:
  US 20040108225A1
• Time in Patent Office: 575 Days
• Field of Search: 422/56, 422/57, 422/58, 422/61, 422/62, 422/68.1, 422/72, 422/82.01, 422/100, 422/102, 436/45, 436/63, 436/177, 436/178, 435/287.2, 435/287.6, 435/287.9, 204/409, 204/400, 204/403.01
• US Class Current: 422/72
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 35/00594 Quality control, including calibration or testing of components of the analyser
• CPC Class Codes: G01N 35/00603 Reinspection of samples
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Automatic diagnostic apparatus includes: a controller for controlling operation of the apparatus and for processing data; a sensing system operably connected to the controller for performing an assay, preferably an electrochemical assay (more preferably an electrochemical immunoassay), of a sample and communicating data from the assay to the controller; voltage supply means for applying a potential differential to the sensing system; and output means for communicating processed data to a user.

Claims

1. An automatic diagnostic apparatus comprising:

a controller for controlling operation of the apparatus and for processing data;

a sample container comprising a substantially frusto-conical outer wall an a substantially conical inner wall, each having a narrow end and a broader end;

the outer wall having a lip at its narrow end and connecting with a subtantially planar annular first base at its broader end;

the substantially conical inner wall connecting at its broader end with inner edge of the annular first base and connecting at its narrow end with a depression;

a rack and platform system including a rotor motor which is operable to spin the sample container to cause heavier components of a sample therein to move toward the first base and lighter components to move up the inner wall to the depression;

a sensing system, which is a syringe and biosensor system comprising electrochemical immunoassay biosensor for performing an electrochemical immunoassay of the lighter components of the sample, and a syringe comprising a syringe body and a plunger effective to draw a sample and, optionally, a reagent in and through the biosensor;

voltage supply means for applying a potential difference to said sensing system; and

output means for communicating processed data to a user.

2. An automatic diagnostic apparatus according to claim 1, wherein:

the sensing system has a motor arm;

the syringe has a base and two ends, one end of which is in communication with the motor arm, and the other end of which internally abuts the syringes base.

3. An automatic diagnostic apparatus according to claim 1, wherein the rack and platform system comprises a block with three shaped apertures for securely holding a reagent cartridge.

4. An automatic diagnostic apparatus according to claim 1 further comprising a reagent cartridge having a substantially planar body with four reagent compartments depending therefrom and having a surface;

the reagent compartments being open at the surface of the planar body.

5. An automatic diagnostic apparatus according to claim 4, wherein the reagent cartridge is filled with a reagent for testing myocardial infarction.

6. An automatic diagnostic apparatus according to claim 4, wherein one of the reagent compartments is filled with alkaline phosphatase, a second reagent compartment is filled with a dried naphthyl phosphate, a third reagent compartment is filled with a wash solution, and the fourth reagent compartment is filled with a buffer solution.

7. An automatic diagnostic apparatus according to claim 1, wherein the rack and platform system is provided with an up/down motor and a forward/backward motor.

8. An automatic diagnostic apparatus according to claim 1, wherein the biosensor comprises a counter electrode, a working electrode contact a biosensor body, a biosensor inlet and a solid phase immunoassay site comprising a porous spacer disk, a porous PVDF disk and a porous graphite disk as a working electrode.

9. An automatic diagnostic apparatus according to claim 8, wherein the porous PDVF disk is impregnated with an antibody.