Methods of using oxidized fungal antigens in antibody testing
First Claim
1. A method of testing an antibody to a fungus in a sample, the method comprising:
- a) producing a fungal antigen suitable for testing an antibody to a fungus, comprising providing a fungal antigen as a culture filtrate, the fungal antigen having not been purified by ion exchange chromatography or isoelectric focusing from the culture filtrate, and contacting the fungal antigen with an oxidizing agent to produce an oxidized fungal antigen suitable for testing an antibody to the fungus;
b) contacting a sample suspected of containing an antibody to a fungus with the oxidized fungal antigen produced in step a) under suitable conditions to allow binding of the antibody, if present in the sample, to the oxidized fungal antigen; and
c) assessing binding between the antibody and the oxidized fungal antigen to determine the presence and/or amount of the antibody in the sample.
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Abstract
The present invention relates to oxidized fungal antigens and methods of making and using thereof. More particularly, the present invention provides a method for producing an oxidized fungal antigen in culture filtrate. The present invention also provides for the produced oxidized fungal antigens. Devices comprising such oxidized fungal antigens, methods for testing for fungal antibodies using the oxidized fungal antigens and methods for producing anti-fungal antibodies using oxidized fungal antigens are further provided. Antigen detection devices comprising anti-fungal antibodies raised against oxidized fungal antigens produced by the present methods are further provided.
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Citations
10 Claims
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1. A method of testing an antibody to a fungus in a sample, the method comprising:
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a) producing a fungal antigen suitable for testing an antibody to a fungus, comprising providing a fungal antigen as a culture filtrate, the fungal antigen having not been purified by ion exchange chromatography or isoelectric focusing from the culture filtrate, and contacting the fungal antigen with an oxidizing agent to produce an oxidized fungal antigen suitable for testing an antibody to the fungus;
b) contacting a sample suspected of containing an antibody to a fungus with the oxidized fungal antigen produced in step a) under suitable conditions to allow binding of the antibody, if present in the sample, to the oxidized fungal antigen; and
c) assessing binding between the antibody and the oxidized fungal antigen to determine the presence and/or amount of the antibody in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification