Modified polynucleotides and uses thereof
First Claim
1. A method for producing a composition comprising a modified poly-ribonucleotide, wherein said modified poly-ribonucleotide is an mRNA, rRNA, or viral RNA, said method comprising the steps of:
- (i) contacting a poly-ribonucleotide in a reaction medium comprising at least 80% v/v organic solvent with a reactant capable of covalently modifying the 2′
-OH position of the ribose rings of said poly-ribonucleotide; and
(ii) reacting said poly-ribonucleotide to produce a modified poly-ribonucleotide comprising greater than 25% of ribose rings bearing a C1-C10 alkanoyl at their 2′
-OH position.
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Accused Products
Abstract
Provided is a polynucleotide comprising mRNA, rRNA or viral RNA, comprising ribose rings that are covalently modified at the 2′-OH position. Further provided are methods for producing a double-stranded oligo- or polynucleotide from a template comprising an oligo- or polyribonucleotide, a proportion of the ribose rings of which are covalently modified at the 2′-OH position to bear a substituent which enables replication of the template by the nucleic acid polymerase. Also provided is use of a poly-nucleotide comprising mRNA, rRNA or viral RNA, a proportion of the ribose rings of which are covalently modified at the 2′-OH position, in a hybridisation reaction.
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Citations
58 Claims
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1. A method for producing a composition comprising a modified poly-ribonucleotide, wherein said modified poly-ribonucleotide is an mRNA, rRNA, or viral RNA, said method comprising the steps of:
- (i) contacting a poly-ribonucleotide in a reaction medium comprising at least 80% v/v organic solvent with a reactant capable of covalently modifying the 2′
-OH position of the ribose rings of said poly-ribonucleotide; and
(ii) reacting said poly-ribonucleotide to produce a modified poly-ribonucleotide comprising greater than 25% of ribose rings bearing a C1-C10 alkanoyl at their 2′
-OH position. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 27, 28, 29, 30, 31)
- (i) contacting a poly-ribonucleotide in a reaction medium comprising at least 80% v/v organic solvent with a reactant capable of covalently modifying the 2′
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26. The method of clain 1, wherein said organic solvent comprises an organic base.
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32. A method for producing a composition comprising a modified poly-ribonucleotide at least 1000 bases in length, said method comprising the steps of:
- (i) contacting a poly-ribonucleotide in a reaction medium comprising at least 80% v/v organic solvent with a reactant capable of covalently modifying the 2′
-OH position of the ribose rings of said poly-ribonucleotide; and
(ii) reacting said poly-ribonucleotide to produce a modified poly-ribonucleotide comprising greater than 25% of ribose rings bearing a C1-C10 alkanoyl at their 2′
-OH position. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
- (i) contacting a poly-ribonucleotide in a reaction medium comprising at least 80% v/v organic solvent with a reactant capable of covalently modifying the 2′
Specification