Multi-color multiplexed analysis in a bio-separation system

• US 6,870,165 B2
• Filed: 10/21/2002
• Issued: 03/22/2005
• Est. Priority Date: 10/19/2001
• Status: Expired due to Term

• Current Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Original Assignee: Biocell Technology LLC
• Inventors: Liu, Ming-Sun, Amirkhanian, Varouj
• Primary Examiner(s): Fulton, Christopher W.
• Assistant Examiner(s): Smith, R. Alexander
• Application Number:
  US10/277,961
• Publication Number:
  US 20030116436A1
• Time in Patent Office: 883 Days
• Field of Search: 250/458.1, 250/459.1, 250/461.1, 250/461.2, 356/317, 356/318, 356/344, 356/417
• US Class Current: 250/458.1
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44782 of a plurality of samples

Abstract

A multi-channel and multi-color bio-separation detection method and apparatus in which a single detector is coupled to a plurality of radiation sources, in a one detector/many radiation sources configuration. Each radiation source directs radiation at a detection zone of a single separation channel, and a single detector is applied to detect light emissions from the detection zones associated with several radiation sources. The radiation sources are activated to direct radiation at the detection zone in a predetermined sequence and further in a cyclic manner, with the detector output synchronized to the radiation sources by a controller. Bio-separation may be conducted simultaneously in all the channels in parallel, with detection time-staggered and/or time multiplexed with respect to the light sources. In one embodiment, low cost light emitting diodes may be used as radiation sources. In another aspect, the detection scheme is configured for radiation induced fluorescence detection in an capillary electrophoresis instrument.

Claims

1. A detection system for a bio-separation device having a separation channel in which bio-separation takes place, comprising:

a detection section along a separation channel defining a single detection zone for analytes;

a plurality of radiation sources;

excitation means for introducing excitation radiations from the radiation sources at the single detection zone as analytes pass the detection zone;

detecting means for detecting radiation emissions;

control means for controlling the radiation sources and detecting means in a manner such that excitation radiations from the radiation sources are introduced at the detection zone in a predetermined sequence and radiation emission from the detection zone associated with each radiation source is detected in a time staggered manner.

2. The detection system as in claim 1, wherein the detecting means comprises a single detector associated with the plurality of radiation sources.

3. The detection section as in claim 1, wherein the control means controls the plurality of radiation sources to activate in successive pulses.

4. The detection section as in claim 3, wherein the control means controls the synchronization of the pulsing of the radiation sources and detection sampling rate and period by taking into account the lag time in emitted radiation for each radiation source, whereby desired detection for a radiation source covers a period when radiation emission is from only one of the radiation sources.

5. The detection system as in claim 4, wherein the control means controls the detecting means to sample the radiation emissions from the separation channels at a rate and period that provide desired radiation emission signal separation between the radiation sources to reduce cross talk.

6. The detection system as in claim 1, wherein the control means controls the detecting means and the radiation sources in a manner to effect detection of radiation emissions associated with the radiation sources in predetermined detection cycles, wherein each detection cycle is repeated at a frequency to provide a desired detection resolution.

7. The detection system as in claim 1, wherein the control means controls the radiation sources and detecting means in a manner to effect detection in a repeated scanning of the radiation sources.

8. The detection system as in claim 1, wherein the radiation sources produce excitation radiations at different wavelength.

9. The detection system as in claim 1 wherein the analytes comprise a material that fluoresces in the presence of the excitation radiation, and the detecting means comprises means for detecting fluorescence emission of the material.

10. The detection system as in claim 1 wherein the radiation emission is at least one of:

fluorescene;

chemiluminescence;

phosphorescence; and

absorbance.

11. A bio-separation instrument, comprising:

a separation channel;

means for separating samples in the separation channel into analytes; and

a detection system, comprising;

a detection section along the separation channel defining a single detection zone for analytes;

a plurality of radiation sources, at least two of the radiation sources have different wavelength;

excitation means for introducing excitation radiations from the radiation sources at the single detection zone as analytes pass the detection zone;

detecting means for detecting radiation emissions;

control means for controlling the radiation sources and detecting means in a manner such that excitation radiations from the excitation sources are introduced at the detection zone in a predetermined sequence and radiation emission from the detection zone associated with each radiation source is detected in a time staggered manner.

12. A bia-separation instrument as in claim 11, wherein the separation channel is defined by a capillary column, and the means for separating a sample is configured to effect separation of the sample by electrophoresis.

13. A method for detecting analytes in a bio-separation device having a separation channel in which bio-separation takes place, comprising the steps of:

defining a single detection zone for analytes along the separation channel;

providing a plurality of radiation sources, at least two of which associat with different wavelengths;

introducing excitation radiations from the plurality of radiation sources at the single detection zone as analytes pass the detection zone;

providing a detector for detecting radiation emissions;

controlling the radiation sources and detector in a manner such that excitation radiations from the radiation sources are introduced at the detection zone in a predetermine sequence and radiation emission from the detection zone associated with each radiation source is detected in a time staggered manner.