Composite material sheet for analysis of substances originating from living body or analogues thereof

• US 6,872,531 B2
• Filed: 05/20/2002
• Issued: 03/29/2005
• Est. Priority Date: 05/18/2001
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Hosoi, Yuichi
• Primary Examiner(s): Goldberg, Jeanine
• Application Number:
  US10/147,860
• Publication Number:
  US 20020172973A1
• Time in Patent Office: 1,044 Days
• Field of Search: 435/287.2, 435/6, 435/7.1, 435/287.1, 536/23.1
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00531 essentially square
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A composite material sheet favorably utilizable for analysis of substances originating from living body or its analogues has partitions two-dimensionally extending on a sheet plane to form on the sheet plane plural fine sections surrounded by the partitions and porous material portions each of which is placed in the fine section, in which the partitions are made of material having a mean density of 0.6 g/cm³ or higher and the porous material portions have a mean density of 1.0 g/cm³ or lower, under the condition that the mean density of material of the partitions is higher than the mean density of the material of the porous material portions.

Claims

1. A composite material sheet comprising partitions two-dimensionally extending on a sheet plane to form on the sheet plane plural fine sections surrounded by the partitions and porous material portions each of which is placed in the fine section, wherein the partitions are made of metal that shields transmission of radioisotope radiation therethrough, and the porous material portions have a mean density of not higher than 1.0 g/cm³, provided that the mean density of material of the partitions is higher than the mean density of the material of the porous material portions wherein the composite material sheet has the fine sections having the porous material portion therein in number of 10 to 10,000 per cm².

2. The composite material sheet of claim 1, wherein the mean density of material of the partitions is in the range of 1 to 20 g/cm³.

3. The composite material sheet of claim 1, wherein the mean density of the porous material portions is in the range of 0.1 to 0.5 g/cm³.

4. The composite material sheet of claim 1, wherein the porous material portions are made of porous organic polymer material.

5. The composite material sheet of claim 1, wherein the fine sections having the porous material portion therein have an opening whose mean area is in the range of 0.001 to 5 mm².

6. The composite material sheet of claim 1, wherein at least one of upper and lower surfaces of the porous material portions is retracted from the upper or lower surface of the adjoining partitions on the sheet plane, so as to enable spotting of a probe solution and prohibit overflow of the solution onto adjacent porous material portions.

7. The composite material sheet of claim 1, wherein probe molecules which can form biochemically specific bonding with substances originating from living body or analogues thereof are attached to the porous material portions.

8. The composite material sheet of claim 7, wherein the probe molecules are oligonucleotides or polynucleotides.

9. A process for detecting complementary nucleic acid fragments which comprises the steps of:

preparing a composite material sheet of claim 1, attaching to each porous material portion a group of single-stranded probe nucleic acid fragments;

bringing single-stranded sample nucleic acid fragments having a radioactive label in a liquid phase into contact with the prepared composite material sheet, whereby single-stranded target nucleic acid fragments in the sample nucleic acid fragments which are complementary to the group of the probe nucleic acid fragments are fixed by hybridization to the probe nucleic acid fragments;

removing unfixed sample nucleic acid fragments from the composite material sheet;

placing the hybridized composite material sheet in contact with a stimulable phosphor sheet so that the stimulable phosphor sheet can absorb and store radiation energy of the radioactive label coming from the fixed nucleic acid fragments;

irradiating the stimulable phosphor sheet with a stimulating light, whereby the stimulable phosphor sheet releases stimulated emissions from the areas in which the radiation energy is stored;

detecting the stimulated emissions photoelectrically to obtain a series of electric signals; and

processing the electric signals to detect the areas in which the complementary nucleic acid fragments are fixed.

10. The process of claim 9, wherein at least one of upper and lower surfaces of the porous material portions in the composite material sheet is retracted from upper or lower surface of the adjoining partitions on the sheet plane.