Nucleic acid detection kits
First Claim
1. A kit comprising:
- a) a first oligonucleotide comprising a 3′
portion and a 5′
portion said 3′
portion completely complementary to a first region of a target nucleic acid wherein said 3′
portion when hybridized to said target nucleic acid forms a hybridized region and said 5′
portion non-complementary to said target nucleic acid; and
b) a second oligonucleotide comprising a 3′
portion and a 5′
portion, said 5′
portion completely complementary to a second region of said target nucleic acid downstream of and contiguous to said first region wherein said 3′
portion extends into said hybridized region; and
c) a structure-specific 5′
-nuclease.
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Accused Products
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
189 Citations
27 Claims
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1. A kit comprising:
-
a) a first oligonucleotide comprising a 3′
portion and a 5′
portion said 3′
portion completely complementary to a first region of a target nucleic acid wherein said 3′
portion when hybridized to said target nucleic acid forms a hybridized region and said 5′
portion non-complementary to said target nucleic acid; and
b) a second oligonucleotide comprising a 3′
portion and a 5′
portion, said 5′
portion completely complementary to a second region of said target nucleic acid downstream of and contiguous to said first region wherein said 3′
portion extends into said hybridized region; and
c) a structure-specific 5′
-nuclease. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification