Nucleic acid detection assays

• US 6,875,572 B2
• Filed: 02/22/2002
• Issued: 04/05/2005
• Est. Priority Date: 01/24/1996
• Status: Expired due to Term

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Third Wave Technologies Incorporated (Hologic Incorporated)
• Inventors: Prudent, James R., Hall, Jeff G., Dahlberg, James E., Lyamichev, Victor I., Brow, Mary Ann D.
• Primary Examiner(s): Siew, Jeffrey
• Application Number:
  US10/081,806
• Publication Number:
  US 20020197623A1
• Time in Patent Office: 1,138 Days
• Field of Search: 435/6, 435/7.1, 435/91.1, 435/91.2, 530/22.1, 530/23.1, 530/24.3, 530/24.31, 530/24.32, 530/24.33
• US Class Current: 435/6.1
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113
• CPC Class Codes: C12Q 1/6823 Release of bound markers
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 2561/109 Invader technology
• CPC Class Codes: C12Q 2563/149 Particles, e.g. beads

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Claims