Process for producing GDP-fucose
First Claim
1. A process for producing guanosine 5′
- -diphosphate-fucose (“
GDP-fucose”
), comprising;
allowing a guanosine 5′
-triphosphate (“
GTP”
) precursor, a saccharide selected from the group consisting of glucose, fructose and mannose, and enzyme sources to be present in an aqueous medium, wherein the enzyme sources are (i) a culture of a microorganism capable of forming GTP from said GTP precursor or a treated product of the culture, and (ii) a culture of a microorganism capable of forming guanosine 5′
-diphosphate-4-keto-6-deoxymannose (“
GKDM”
) from said saccharide and GTP or a treated product of the culture;
forming and accumulating GKDM in the aqueous medium; and
then adding a culture of a microorganism capable of converting GKDM into GDP-fucose or a treated product of the culture, as an enzyme source, to the aqueous medium to convert the accumulated GKDM into GDP-fucose;
forming and accumulating GDP-fucose in the aqueous medium; and
recovering the GDP-fucose from the aqueous medium, wherein the treated products of the culture are treated products independently selected from the group consisting of a concentrated product of the culture, a dried product of the culture, cells obtained by centrifuging the culture, a dried product of the cells, a freeze-dried product of the cells, a surfactant-treated product of the cells, a solvent-treated product of the cells, an enzyme-treated product of the cells and an immobilized product of the cells.
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Abstract
Processes for producing GDP-fucose, comprising allowing GKDM and a culture broth of a microorganism capable of converting GKDM into GDP-fucose to be present in an aqueous medium, forming and accumulating GDP-fucose therein, and recovering the GDP-fucose therefrom; or comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable of forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, converting the accumulated GKDM into GDP-fucose using as a culture broth of a microorganism capable of converting GKDM into GDP-fucose to form and accumulate GDP-fucose therein, and recovering the GDP-fucose therefrom; and a process for producing GKDM, comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, and recovering the GKDM therefrom.
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Citations
25 Claims
-
1. A process for producing guanosine 5′
- -diphosphate-fucose (“
GDP-fucose”
), comprising;allowing a guanosine 5′
-triphosphate (“
GTP”
) precursor, a saccharide selected from the group consisting of glucose, fructose and mannose, and enzyme sources to be present in an aqueous medium, wherein the enzyme sources are (i) a culture of a microorganism capable of forming GTP from said GTP precursor or a treated product of the culture, and (ii) a culture of a microorganism capable of forming guanosine 5′
-diphosphate-4-keto-6-deoxymannose (“
GKDM”
) from said saccharide and GTP or a treated product of the culture;
forming and accumulating GKDM in the aqueous medium; and
thenadding a culture of a microorganism capable of converting GKDM into GDP-fucose or a treated product of the culture, as an enzyme source, to the aqueous medium to convert the accumulated GKDM into GDP-fucose;
forming and accumulating GDP-fucose in the aqueous medium; and
recovering the GDP-fucose from the aqueous medium, wherein the treated products of the culture are treated products independently selected from the group consisting of a concentrated product of the culture, a dried product of the culture, cells obtained by centrifuging the culture, a dried product of the cells, a freeze-dried product of the cells, a surfactant-treated product of the cells, a solvent-treated product of the cells, an enzyme-treated product of the cells and an immobilized product of the cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- -diphosphate-fucose (“
-
15. A process for producing guanosine 5′
- -diphospho-fucose (“
GDP-fucose”
), comprising;allowing a guanosine 5′
-triphosphate (“
GTP”
), a saccharide selected from the group consisting of glucose, fructose and mannose, and an enzyme source to be present in an aqueous medium, wherein the enzyme source is a culture of a microorganism capable of forming guanosine 5′
-diphosphate-4-keto-6-deoxymannose (“
GKDM”
) from said saccharide and GTP or a treated product of the culture;
forming and accumulating GKDM in the aqueous medium; and
thenadding a culture of a microorganism capable of converting GKDM into GDP-fucose or a treated product of the culture, as an enzyme source, to the aqueous medium to convert the accumulated GKDM into GDP-fucose;
forming and accumulating GDP-fucose in the aqueous medium; and
recovering the GDP-fucose from the aqueous medium, wherein the treated products of the culture are treated products independently selected from the group consisting of a concentrated product of the culture, a dried product of the culture, cells obtained by centrifuging the culture, a dried product of the cells, a freeze-dried product of the cells, a surfactant-treated product of the cells, a solvent-treated product of the cells, an enzyme-treated product of the cells and an immobilized product of the cells. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
- -diphospho-fucose (“
Specification