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Process for producing GDP-fucose

  • US 6,875,591 B1
  • Filed: 08/03/2000
  • Issued: 04/05/2005
  • Est. Priority Date: 08/10/1999
  • Status: Expired due to Fees
First Claim
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1. A process for producing guanosine 5′

  • -diphosphate-fucose (“

    GDP-fucose”

    ), comprising;

    allowing a guanosine 5′

    -triphosphate (“

    GTP”

    ) precursor, a saccharide selected from the group consisting of glucose, fructose and mannose, and enzyme sources to be present in an aqueous medium, wherein the enzyme sources are (i) a culture of a microorganism capable of forming GTP from said GTP precursor or a treated product of the culture, and (ii) a culture of a microorganism capable of forming guanosine 5′

    -diphosphate-4-keto-6-deoxymannose (“

    GKDM”

    ) from said saccharide and GTP or a treated product of the culture;

    forming and accumulating GKDM in the aqueous medium; and

    then adding a culture of a microorganism capable of converting GKDM into GDP-fucose or a treated product of the culture, as an enzyme source, to the aqueous medium to convert the accumulated GKDM into GDP-fucose;

    forming and accumulating GDP-fucose in the aqueous medium; and

    recovering the GDP-fucose from the aqueous medium, wherein the treated products of the culture are treated products independently selected from the group consisting of a concentrated product of the culture, a dried product of the culture, cells obtained by centrifuging the culture, a dried product of the cells, a freeze-dried product of the cells, a surfactant-treated product of the cells, a solvent-treated product of the cells, an enzyme-treated product of the cells and an immobilized product of the cells.

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